FGF 21 Mouse, Sf9

Fibroblast Growth Factor-21 Mouse Recombinant, Sf9
Cat. No.
BT7299
Source
Sf9, Baculovirus cells.
Synonyms
Fibroblast growth factor 21, FGF-21.
Appearance
Sterile Filtered colorless clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
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Description

FGF-21 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 190 amino acids (29-210a.a.) and having a molecular mass of 21.0kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).
FGF21 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
The FGFs are a family of more than 20 small (approximately 17–26 kDa) secreted peptides. These proteins were initially characterized by their ability to stimulate fibroblast proliferation. This mitogenic activity is mediated through FGF receptors (FGFRs) 1, 2, or 3. A fourth closely related tyrosine kinase receptor (FGFR4) was able to bind the FGFs but did not lead to a mitogenic response. FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors (FGFRs): FGFR-1, -2, -3, and -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, and FGFR-5, which lacks an intracellular kinase domain. Evidence is growing that FGFRs can be important for regulation of glucose and lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in β cells leads to diabetes in mice, implying that proper FGF signaling is required for normal β cell function and maintenance of glycemia. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism and bile acid synthesis. FGF-21 is preferentially expressed in the liver, but exact knowledge of FGF-21 bioactivity and its mode of action has been lacking to date. FGF-21 is a potent activator of glucose uptake in adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, and lowers blood glucose and triglyceride levels when therapeutically administered to diabetic rodents.
Description
FGF-21 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 190 amino acids (29-210a.a.) and having a molecular mass of 21.0 kDa. Molecular size on SDS-PAGE will appear at approximately 18-28 kDa. FGF21 is expressed with an 8 amino acid His tag at the C-Terminus and purified by proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless clear solution.
Formulation
FGF-21 protein solution (0.5 mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if the entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Synonyms
Fibroblast growth factor 21, FGF-21.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY ASLEHHHHHH.

Product Science Overview

Structure and Production

FGF-21 is a single, non-glycosylated polypeptide chain containing 183 amino acids, including an N-terminal methionine, with a molecular mass of approximately 20.1 kDa . The recombinant version of FGF-21, produced in Escherichia coli, is purified using proprietary chromatographic techniques . This recombinant protein is typically provided as a filtered, lyophilized powder, which can be reconstituted in deionized water to prepare a working stock solution .

Biological Functions

FGF-21 is predominantly expressed in the liver but also found in other tissues. It plays a crucial role in regulating glucose and lipid metabolism. Some of its key functions include:

  • Glucose Uptake: FGF-21 is a potent activator of glucose uptake in adipocytes, making it a valuable agent in managing blood glucose levels .
  • Obesity Protection: When overexpressed in transgenic mice, FGF-21 protects against diet-induced obesity .
  • Metabolic Regulation: Therapeutic administration of FGF-21 in diabetic rodents has been shown to lower blood glucose and triglyceride levels .
Therapeutic Potential

FGF-21 has shown promise as a therapeutic agent for metabolic disorders. It has been used in phase II clinical trials for treating metabolic diseases, which are prevalent worldwide . The ability of FGF-21 to improve glucose and lipid profiles, along with its potential to induce weight loss, underscores its significant market potential .

Industrial Production

The production of recombinant FGF-21 must be efficient to meet market demand. Recent advancements in microbial industrial fermentation have led to high-yield production of FGF-21 . Techniques such as the double promoter and tandem gene strategy have significantly improved the expression levels of FGF-21 in engineered strains . These advancements lay a solid foundation for the future industrialization of FGF-21 and its application in various therapeutic contexts .

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