FGF 21 Bovine
(a) Analysis by Gel Filtration.
(b) Analysis by SDS-PAGE.
Description
The FGF-21 is purified by proprietary chromatographic techniques.
Product Specs
The FGF family comprises over 20 small (~17–26 kDa) secreted peptides, initially recognized for their ability to promote fibroblast proliferation. This mitogenic effect was found to be mediated by FGF receptors (FGFRs) 1, 2, or 3. A related receptor, FGFR4, displayed binding affinity for FGFs but did not elicit a mitogenic response.
FGFs exert their effects on cellular activity through at least five distinct subfamilies of high-affinity FGFRs: FGFR-1, -2, -3, and -4, all possessing intrinsic tyrosine kinase activity (with multiple splice isoforms except for FGFR-4), and FGFR-5, which lacks an intracellular kinase domain. Emerging evidence suggests a role for FGFRs in regulating glucose and lipid homeostasis. Overexpression of a dominant-negative FGFR-1 in pancreatic beta cells results in diabetes in mice, highlighting the importance of FGF signaling for normal beta cell function and glucose control. FGFR-2 appears crucial for pancreatic development, while FGFR-4 has been implicated in cholesterol metabolism and bile acid synthesis.
FGF-19 has demonstrated the ability to induce resistance to diet-induced obesity and improve glucose and lipid profiles in diabetic rodents, potentially due to its influence on metabolic rates, suggesting a role as a regulator of energy expenditure.
While FGF-21 exhibits preferential expression in the liver, a comprehensive understanding of its bioactivity and mechanism of action has been limited. It is known to be a potent activator of glucose uptake in adipocytes, providing protection against diet-induced obesity when overexpressed in transgenic mice, and reducing blood glucose and triglyceride levels upon therapeutic administration to diabetic rodents.
(a) Gel Filtration Analysis.
(b) SDS-PAGE Analysis.
1. UV spectroscopy at 280 nm, employing an absorbance value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is determined using the PC GENE computer analysis program for protein sequences (IntelliGenetics).
2. RP-HPLC analysis, utilizing a standard solution of Recombinant FGF-21 as a reference standard.
Product Science Overview
Fibroblast Growth Factor-21 Bovine Recombinant (FGF-21 Bovine) is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain containing 182 amino acids, with a molecular weight of approximately 19.5 kDa . The protein is purified using proprietary chromatographic techniques to ensure its purity and stability .
FGF-21 is primarily expressed in the liver and has been identified as a potent activator of glucose uptake in adipocytes. It plays a crucial role in regulating glucose and lipid homeostasis, protecting against diet-induced obesity, and lowering blood glucose and triglyceride levels when administered therapeutically . Additionally, FGF-21 has shown potential in improving metabolic profiles in diabetic rodents .
Recombinant bovine FGF-21 has been widely used in various research and therapeutic applications. It has demonstrated efficacy in promoting wound healing, reducing hemorrhage, and facilitating tissue repair and regeneration . The protein’s ability to stimulate the repair and regeneration of tissues derived from the mesoderm and ectoderm makes it valuable in the management of burns, fresh wounds, and chronic wounds .
Lyophilized FGF-21 Bovine Recombinant is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term preservation . It is recommended to reconstitute the lyophilized protein in sterile water or a solution containing 0.4% NaHCO3, ensuring a concentration of not less than 100 µg/ml .
