FGF 1 Mouse, His
Description
FGF-1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Fibroblast Growth Factors (FGFs) are a family of heparin-binding proteins that play crucial roles in various cellular processes, including development, repair, and maintenance of tissues. Among these, Fibroblast Growth Factor-acidic (FGF-1), also known as Heparin-Binding Growth Factor-1 (HBGF-1), is a significant member due to its diverse biological functions.
FGF-1 is a single-chain protein with a molecular weight ranging from 15 to 25 kDa. It possesses a conserved core of approximately 120 amino acids. The recombinant version of FGF-1, specifically the mouse recombinant form with a His Tag, is engineered to facilitate purification and detection. The His Tag is a sequence of histidine residues added to the protein, which allows for easy purification using metal affinity chromatography.
FGF-1 is involved in numerous cellular activities:
- Mitogenesis: It stimulates cell division, particularly in mesenchymal, neuroectodermal, and endothelial cells.
- Angiogenesis: FGF-1 promotes the formation of new blood vessels, which is essential for wound healing and tissue regeneration.
- Wound Healing: It accelerates the repair of damaged tissues by promoting cell proliferation and migration.
- Differentiation: FGF-1 influences the differentiation of various cell types, contributing to tissue development and maintenance.
FGF-1 exerts its effects by binding to high-affinity FGF receptors (FGFRs) on the surface of target cells. This binding triggers a signal transduction cascade involving kinases and transcription factors, leading to the activation of various cellular processes. The interaction with heparin sulfate proteoglycans (HSPGs) further enhances the stability and activity of FGF-1.
Recombinant FGF-1, including the mouse recombinant form with a His Tag, is widely used in biomedical research. Some key applications include:
- Cell Culture: FGF-1 is used to maintain, expand, and differentiate various cell types in culture.
- Stem Cell Research: It plays a crucial role in the maintenance and differentiation of pluripotent stem cells.
- Tissue Engineering: FGF-1 is employed in the development of engineered tissues for regenerative medicine.
- Signaling Pathway Studies: Researchers use FGF-1 to investigate the signaling pathways involved in cell growth, differentiation, and survival.
