FGF 1 Mouse
Greater than 95.0% as determined by SDS-PAGE.
Description
Fibroblast Growth Factor-acidic Mouse Recombinant (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15.9kDa.
The FGF acidic is purified by proprietary chromatographic techniques.
Product Specs
Acidic fibroblast growth factor (FGF-1) belongs to the FGF family, known for their roles in cell growth, survival, and various biological processes. FGFs contribute to embryonic development, tissue repair, and angiogenesis (blood vessel formation). FGF-1 specifically influences endothelial cell activity, crucial for blood vessel formation. It acts as a mitogen, promoting cell division in various cell types derived from mesoderm and neuroectoderm, highlighting its role in organ development. Multiple forms of FGF-1 exist due to alternative splicing, leading to variations in its protein structure. These growth factors stimulate cell growth in various tissues and are potent mitogens for different cell types in laboratory settings. The distribution and concentration of these growth factors vary across tissues.
Recombinant Mouse Fibroblast Growth Factor-acidic (FGF-1), expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 141 amino acids. This protein has a molecular weight of 15.9 kDa.
FGF-acidic is purified using specialized chromatographic techniques.
Lyophilized from a 0.2 µm filtered concentrated solution in phosphate-buffered saline (PBS) at pH 7.5.
Purity is determined to be greater than 95.0% as assessed by SDS-PAGE analysis.
The ED50, determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is less than 0.2 ng/ml. This corresponds to a Specific Activity of 5 x 106 IU/mg.
MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.
Product Science Overview
FGF-acidic is a single-chain protein with a molecular weight of approximately 15.8 kDa . It binds to and activates high-affinity FGF receptors (FGFR1-4) on target cells, initiating a signal transduction cascade involving kinases and transcription factors . This activation leads to several cellular effects, such as:
FGF-acidic is highly regulated and acts as a direct angiogenesis factor . It is known for its mitogenic activity, particularly targeting endothelial cells . However, unlike some other FGFs, FGF-acidic lacks a signal peptide segment, meaning it is not secreted via the endoplasmic reticulum (ER) and Golgi bodies .
Due to its role in angiogenesis, FGF-acidic is considered a potential oncogene. Its overexpression is closely associated with several cancers, including those affecting the eyes, joints, and pancreas . Understanding the regulation and function of FGF-acidic can provide insights into therapeutic targets for these diseases.
