FGF 1 Rat

Fibroblast Growth Factor-Acidic Rat Recombinant
Cat. No.
BT5982
Source
Escherichia Coli.
Synonyms

Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, aFGF, binding growth factor 1, HBGF-1, Fgf1, Fgfa, HBGF1.

Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Fibroblast Growth Factor-acidic Rat Recombinant (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15.9 kDa.
The FGF acidic is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Acidic fibroblast growth factor (aFGF or FGF-1) is a member of the fibroblast growth factor (FGF) family. FGFs have broad mitogenic and cell survival activities and play crucial roles in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, and tumor growth and invasion. aFGF, in particular, is known for its role in modifying endothelial cell migration and proliferation, making it a crucial angiogenic factor. It exhibits mitogenic activity on a variety of mesoderm- and neuroectoderm-derived cells in vitro, suggesting its involvement in organogenesis. Notably, there are three identified alternatively spliced variants of aFGF, resulting in different isoforms. The binding growth factors associated with aFGF demonstrate angiogenic properties in vivo and act as potent mitogens for a range of cell types in vitro. However, it's important to note that there are variations in the tissue distribution and concentration of these growth factors.
Description
Fibroblast Growth Factor-acidic Rat Recombinant (FGF-1), produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 141 amino acids. It has a molecular mass of 15.9 kDa. The purification process for FGF acidic involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder
Formulation
Lyophilized at a concentration of 1 mg/ml in 5mM Na2PO4, pH 7.5, and 50mM NaCl.
Solubility
To reconstitute the lyophilized Fibroblast Growth Factor-acidic, it is recommended to dissolve it in sterile 18MΩ-cm H2O at a concentration not less than 100 µg/ml. This reconstituted solution can then be further diluted into other aqueous solutions as needed.
Stability
Lyophilized Fibroblast Growth Factor-1, while stable at room temperature for up to 3 weeks, should ideally be stored desiccated at a temperature below -18°C. Upon reconstitution, FGF-a should be stored at 4°C for a period of 2-7 days. For long-term storage, it is recommended to keep it at -18°C. Avoid repeated freeze-thaw cycles.
Purity
Greater than 98.0% as determined by SDS-PAGE analysis.
Biological Activity
The ED50, determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is less than 0.2 ng/ml. This corresponds to a Specific Activity of 5 x 10^6 IU/mg.
Synonyms

Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, aFGF, binding growth factor 1, HBGF-1, Fgf1, Fgfa, HBGF1.

Source
Escherichia Coli.
Amino Acid Sequence
MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.

Product Science Overview

Introduction

Fibroblast Growth Factors (FGFs) are a family of cell-signaling proteins involved in a wide range of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, and angiogenesis . Among the FGFs, Fibroblast Growth Factor-Acidic (FGF-1), also known as Heparin-Binding Growth Factor-1 (HBGF-1), is one of the most studied members due to its significant role in cellular functions .

Structure and Biochemical Properties

FGF-1 is a single-chain protein with a molecular weight ranging from 15 to 25 kDa . It possesses a conserved core of about 120 amino acids and is characterized by its ability to bind heparin and heparan sulfate . This binding property allows FGF-1 to be sequestered in the extracellular matrix and released upon tissue injury or remodeling .

Functions and Mechanisms

FGF-1 functions by binding to and activating high-affinity FGF receptors (FGFRs) on target cells . This interaction triggers a signal transduction cascade involving kinases and transcription factors, leading to various cellular effects such as mitogenesis, differentiation, migration, and angiogenesis . FGF-1 is particularly known for its role in wound healing and tissue repair .

Applications in Biomedical Research

Recombinant FGF-1, including rat recombinant FGF-1, is widely used in biomedical research. It is employed in cell culture applications to maintain, expand, and differentiate various cell types . For instance, FGF-1 is used to study signaling interactions and pathways involved in cell growth and development .

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