FCGR2A Human, HEK
HEK293 Cells.
CD32, CD32A, CDw32, FcGR, IGFR2, MGC23887, MGC30032, Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, Fc-gamma-RIIa, FcRII-a, FCG2, FCGR2A1.
Greater than 95.0% as determined by SDS-PAGE.
Description
FCGR2A Human Recombinant is a single, glycosylated, polypeptide chain (30-218 a.a) containing a total of 195 amino acids, having a molecular mass of 21.6kDa.
FCGR2A is fused to a 6 amino acid His-tag at C-terminus and is purified by proprietary chromatographic techniques.
Product Specs
Recombinant human FCGR2A is a glycosylated polypeptide chain encompassing amino acids 30 to 218, resulting in a total of 195 amino acids. Its molecular mass is 21.6 kDa. For purification purposes, a 6-amino acid His-tag is fused to the C-terminus, and proprietary chromatographic techniques are employed.
The FCGR2A protein is provided at a concentration of 1 mg/ml in a solution containing 10% glycerol and phosphate-buffered saline (pH 7.4).
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended periods, storage at -20°C in a frozen state is recommended. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Multiple freeze-thaw cycles should be avoided.
The purity of the product is greater than 95.0%, as determined by SDS-PAGE analysis.
The ED50 value, determined through a functional ELISA utilizing Human IgG1 Fc (CAT# pro-2763), is less than or equal to 5 ug/ml, indicating its binding capability.
CD32, CD32A, CDw32, FcGR, IGFR2, MGC23887, MGC30032, Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, Fc-gamma-RIIa, FcRII-a, FCG2, FCGR2A1.
HEK293 Cells.
SADSQAAAPP KAVLKLEPPW INVLQEDSVT LTCQGARSPE SDSIQWFHNG NLIPTHTQPS YRFKANNNDS GEYTCQTGQT SLSDPVHLTV LSEWLVLQTP HLEFQEGETI MLRCHSWKDK PLVKVTFFQN GKSQKFSHLD PTFSIPQANH SHSGDYHCTG NIGYTLFSSK PVTITVQVPS MGSSSPMGIH HHHHH.
Product Science Overview
CD32a is a cell surface receptor predominantly expressed on phagocytic cells such as macrophages and neutrophils . The gene encoding CD32a is part of a family of genes that includes other Fc receptors like FcγRIIb and FcγRIIc. These receptors are encoded by mRNA splice variants of three highly related genes: FCGR2A, FCGR2B, and FCGR2C .
The recombinant form of CD32a, expressed in HEK293 cells (Human Embryonic Kidney 293 cells), is often used in research to study its function and interactions. The recombinant protein typically includes a His-tag for purification purposes and is lyophilized for storage .
CD32a plays a significant role in the immune system by mediating various immune responses. It is involved in:
- Phagocytosis: The process by which phagocytic cells engulf and digest pathogens and debris.
- Clearing Immune Complexes: CD32a helps in the removal of immune complexes from the bloodstream, preventing their deposition in tissues and subsequent inflammation .
- Signal Transduction: CD32a can initiate signaling cascades that lead to various cellular responses, including cytokine production and cell activation .
The study of CD32a is important for understanding its role in various diseases and therapeutic applications. For instance, CD32a is implicated in autoimmune diseases, where its interaction with immune complexes can contribute to inflammation and tissue damage. Additionally, targeting CD32a with therapeutic antibodies is being explored as a potential treatment for certain cancers and inflammatory conditions .
Recombinant CD32a expressed in HEK293 cells is widely used in research to:
- Investigate Fc Receptor Functions: Understanding how CD32a interacts with antibodies and other immune components.
- Develop Therapeutic Antibodies: Designing antibodies that can modulate the activity of CD32a for therapeutic purposes.
- Study Immune Responses: Exploring the role of CD32a in various immune processes and its impact on disease progression .
