FAM3D Human
Family with Sequence Similarity 3, Member D Human Recombinant
Cat. No.
BT8018
Source
Escherichia Coli.
Synonyms
Protein FAM3D, FAM3D, EF7, OIT1.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
FAM3D Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 26-224) containing 209 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 23.3kDa (calculated).
Product Specs
Introduction
Belonging to the FAM3 family, Family with Sequence Similarity 3, Member D (FAM3D) has been associated with narcolepsy and diabetes mellitus in pancreatic adenocarcinoma. Despite these links, the precise function of FAM3D is still not fully understood. FAM3D is highly expressed in the placenta and shows low expression levels in the small intestine.
Description
FAM3D Human Recombinant, produced in E.Coli, is an un-glycosylated polypeptide chain with a single chain. It consists of amino acids 26-224 (a.a), totaling 209 amino acids. This includes a 10 a.a N-terminal His tag, resulting in a calculated total molecular mass of 23.3kDa.
Physical Appearance
White powder, lyophilized and filtered.
Formulation
FAM3D is supplied as a lyophilized powder, filtered to 0.4 µm, from a solution of 0.5mg/ml in 0.05M Acetate buffer at pH 4.0.
Solubility
To create a working stock solution of around 0.5mg/ml, we recommend adding 0.1M Acetate buffer at pH 4 to the lyophilized pellet. Allow the pellet to fully dissolve at 37°C. If a higher pH value is needed, dilute the solution extensively with the appropriate buffer to a concentration of 10µg/ml. The solubility of this antigen is limited at higher concentrations. Note: FAM3D is not sterile. Before using in cell culture, the product should be filtered through a suitable sterile filter.
Stability
The lyophilized protein should be stored at -20°C. Once reconstituted, aliquot the product to prevent repeated cycles of freezing and thawing. The reconstituted protein remains stable at 4°C for a limited duration and shows no noticeable change for up to two weeks when stored at this temperature.
Purity
The purity is determined to be greater than 90.0% using SDS-PAGE analysis.
Synonyms
Protein FAM3D, FAM3D, EF7, OIT1.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHASYMSFSMKTIR LPRWLAASPT KEIQVKKYKC GLIKPCPANY FAFKICSGAA NVVGPTMCFE DRMIMSPVKN NVGRGLNIAL VNGTTGAVLG QKAFDMYSGD VMHLVKFLKE IPGGALVLVA SYDDPGTKMN DESRKLFSDL GSSYAKQLGF RDSWVFIGAK DLRGKSPFEQ FLKNSPDTNK YEGWPELLEM EGCMPPKPF.
Product Science Overview
Gene and Protein Information
- Gene Symbol: FAM3D
- Other Names: FAM3 Metabolism Regulating Signaling Molecule D, Oncoprotein-Induced Transcript 1 (OIT1), Cytokine-Like Protein EF-7
- Gene ID: 131177
- Chromosomal Location: Chromosome 3
- Protein ID: Q96BQ1
Function and Pathways
FAM3D is associated with several biological pathways, including those related to pancreatic cancer subtypes. The protein encoded by FAM3D has cytokine activity, which means it can act as a signaling molecule in the immune system. It plays a crucial role in the regulation of metabolic processes, particularly in the context of insulin secretion .
Clinical Significance
Mutations or dysregulation of the FAM3D gene have been linked to various diseases, including:
- Epilepsy, Idiopathic Generalized 5
- Dystonia, Dopa-Responsive
These associations highlight the importance of FAM3D in maintaining normal physiological functions and its potential role in disease mechanisms .
Research and Applications
Summary
FAM3D is a significant protein-coding gene with roles in metabolic regulation and immune signaling. Its involvement in critical pathways and diseases makes it a valuable target for research and therapeutic applications.
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