FABP1 Mouse, His
Fatty acid-binding protein 1 liver, L-FABP, FABPL, FABP-1, FABP1, Z-protein, Fatty acid-binding protein, liver.
Greater than 95.0% as determined by SDS-PAGE.
Description
FABP1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 150 amino acids (1-127 a.a.) and having a molecular mass of 16.6 kDa. The FABP1 is fused to a 23 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Fatty acid-binding protein 1 liver, L-FABP, FABPL, FABP-1, FABP1, Z-protein, Fatty acid-binding protein, liver.
MGSSHHHHHH SSGLVPRGSH MGSMNFSGKY QLQSQENFEP FMKAIGLPED LIQKGKDIKG VSEIVHEGKK IKLTITYGPK VVRNEFTLGE ECELETMTGE KVKAVVKLEG DNKMVTTFKG IKSVTELNGD TITNTMTLGD IVYKRVSKRI.
Product Science Overview
Fatty Acid Binding Protein-1 (FABP1), also known as liver-type fatty acid-binding protein (L-FABP), is a member of the fatty acid-binding protein family. These proteins are involved in the binding, transport, and metabolism of long-chain fatty acids (LCFAs) and other hydrophobic molecules. FABP1 is primarily expressed in the liver but can also be found in the intestine, kidney, pancreas, stomach, and lung .
The fatty acid-binding proteins were initially discovered in 1972 through experiments using labeled oleate to identify a soluble fatty acid carrier in the enterocyte responsible for intestinal absorption of LCFAs . FABP1 has a unique structure compared to other members of the FABP family, allowing it to bind multiple ligands simultaneously. It has a larger solvent-accessible core, which enables more diverse substrate binding .
FABP1 plays a significant role in the binding and transport of LCFAs, endocannabinoids, phytocannabinoids, and other hydrophobic molecules. It is involved in various metabolic processes, including fatty acid uptake, transport, and metabolism. FABP1 is also known to bind bilirubin, monoglycerides, bile acids, and fatty acyl CoA . This protein helps prevent cytotoxicity by binding potentially toxic molecules when unbound .
The His tag is a sequence of histidine residues added to proteins to facilitate their purification and detection. Recombinant proteins, such as the mouse FABP1 with a His tag, are produced using genetic engineering techniques. These proteins are expressed in host cells, such as Escherichia coli, and purified using affinity chromatography methods that exploit the His tag .
Recombinant mouse FABP1 with a His tag is used in various research applications, including studies on fatty acid metabolism, liver function, and the role of FABP1 in metabolic diseases. The His tag allows for easy purification and detection of the protein, making it a valuable tool for researchers .
