EXOSC10 Human

EXOSC10 is a nucleolar protein and a key component of the exosome complex . The exosome complex itself is composed of multiple exoribonucleases, which are enzymes that degrade RNA molecules from the 3’ end . EXOSC10 specifically is known to have exoribonuclease activity, contributing to the overall function of the exosome complex .

The primary function of EXOSC10 is to participate in the degradation of unstable mRNAs containing AU-rich elements (AREs) within their 3’ untranslated regions . It is also involved in the 3’ processing of the 7S pre-rRNA to the mature 5.8S rRNA . Additionally, EXOSC10 plays a role in the replication-dependent histone mRNA degradation .

EXOSC10 is essential for maintaining RNA stability and processing within the cell. It is involved in several critical cellular processes, including:

• mRNA Surveillance: EXOSC10 is part of a post-splicing multiprotein complex involved in mRNA nuclear export and mRNA surveillance . It helps in the nonsense-mediated decay (NMD) of mRNAs containing premature stop codons .
• rRNA Processing: It is required for the proper processing of rRNA, which is essential for ribosome biogenesis and protein synthesis .
• Histone mRNA Degradation: EXOSC10 plays a role in the degradation of histone mRNAs, which is crucial for cell cycle regulation .

Recombinant Human Exosome Component 10 is produced using an in vitro wheat germ expression system . This method helps preserve the correct conformational folding necessary for its biological function . The recombinant protein typically includes a GST-tag at the N-terminal, corresponding to the amino acids 1-885 of the full-length human EXOSC10 .

Recombinant EXOSC10 is used in various research applications, including:

• ELISA: For detecting and quantifying specific proteins.
• Western Blot: For analyzing protein expression.
• SDS-PAGE: For separating proteins based on their molecular weight.
• Affinity Purification: For isolating specific proteins from a mixture .

Recombinant EXOSC10 should be stored at -80°C to maintain its stability and avoid freeze-thaw cycles . It is typically supplied in a buffer containing 50 mM Tris-HCl and 10 mM reduced Glutathione, pH 8.0 .