EPO, Clone PAT1C12AT
Description
Product Specs
Erythropoietin (EPO) belongs to the EPO/TPO family of cytokines. This secreted, glycosylated protein consists of 4 alpha helical bundles. EPO, present in plasma, plays a crucial role in red blood cell production. It does so by promoting the differentiation of erythroid cells and initiating the synthesis of hemoglobin. Moreover, EPO exhibits neuroprotective effects against various brain injuries and possesses anti-apoptotic properties in multiple tissue types.
The product is provided at a concentration of 1mg/ml and is dissolved in a solution composed of PBS with a pH of 7.4, 10% Glycerol, and 0.02% Sodium Azide.
For storage durations of up to 1 month, maintain the product at a temperature of 4°C. If longer storage is required, store the product at -20°C. It is crucial to avoid subjecting the product to repeated cycles of freezing and thawing.
EPO antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT1C12AT.
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
Clone PAT1C12AT is a monoclonal antibody specifically designed to target human erythropoietin. This clone is derived from the hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with human EPO . The antibody is of the IgG1 subclass, with a heavy chain and kappa light chain .
The production of the EPO antibody involves the hybridoma technique, where the mouse myeloma cells are fused with the spleen cells of immunized mice. This results in the creation of hybrid cells that can produce the desired antibody. The antibody is then purified from mouse ascitic fluids using protein-A affinity chromatography .
The EPO antibody is supplied as a sterile filtered colorless solution, typically containing phosphate-buffered saline (PBS) with a pH of 7.4, 10% glycerol, and 0.02% sodium azide . For optimal stability, the antibody should be stored at 4°C for short-term use (up to 1 month) and at -20°C for long-term storage to prevent freeze-thaw cycles .
The EPO antibody clone PAT1C12AT is widely used in various research applications, including:
- Western Blotting: For detecting EPO in different samples.
- Immunoaffinity Purification: For purifying EPO from complex mixtures.
- Immunohistochemistry: For studying the localization of EPO in tissues.
The EPO antibody clone PAT1C12AT is significant in both clinical and research settings. It helps in understanding the role of EPO in erythropoiesis and its potential therapeutic applications. Additionally, EPO has neuroprotective activity against various brain injuries and antiapoptotic functions in several tissue types .
