Epo (NYRhEPO) Antibody

Erythropoietin Mouse Anti Human, (NYRhEPO)
Cat. No.
BT4538
Source
Synonyms
EPO-a, EPO-alpha, Epoetin, EP, MGC138142.
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Product Specs

Introduction
As a member of the EPO/TPO family, this gene encodes a secreted, glycosylated cytokine with a four alpha helical bundle structure. This cytokine, present in plasma, plays a crucial role in red blood cell production by stimulating erythroid differentiation and initiating hemoglobin synthesis. Moreover, it exhibits neuroprotective properties against various brain injuries and demonstrates antiapoptotic functions in diverse tissues.
Formulation
The antibody is provided at a concentration of 1 mg/ml in phosphate-buffered saline (PBS) after reconstitution.
Shipping Conditions
The antibody is shipped in a lyophilized state at room temperature.
Storage Procedures
For long-term storage, keep the lyophilized antibody at 4°C in a dry environment. After reconstitution, aliquot the antibody and store it at -20°C if not intended for use within one month.
Solubility
Reconstitute the antibody using sterile water. Ensure gentle mixing, rinsing the vial sides, and allowing 30-60 seconds for complete reconstitution before use.
Applications
This antibody is suitable for use in various applications, including direct enzyme-linked immunosorbent assay (ELISA), Western blot analysis, and immunoprecipitation.
Titer
In direct ELISA, a 1:10,000 dilution of the antibody exhibits an optical density (O.D.) of 0.5 when using alkaline phosphatase-conjugated rabbit anti-mouse immunoglobulin G (Jackson Laboratories).
Synonyms
EPO-a, EPO-alpha, Epoetin, EP, MGC138142.
Purification Method
Ion exchange.
Type
Mouse Anti Human Monoclonal.
Clone
NYRhEPO.
Immunogen
r.Human erythropoietin.
Ig Subclass
Mouse IgG1.

Product Science Overview

Structure and Function

Erythropoietin is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha-helical bundles . This protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis . Additionally, EPO has neuroprotective activity against various potential brain injuries and anti-apoptotic functions in several tissue types .

Erythropoietin Mouse Anti Human (NYRhEPO)

The Erythropoietin Mouse Anti Human (NYRhEPO) is a monoclonal antibody specifically designed to target human erythropoietin. This antibody is produced by immunizing mice with recombinant human erythropoietin, resulting in the generation of a specific immune response. The resulting monoclonal antibody is then purified and characterized for use in various research applications.

Clone and Immunogen: The clone used for this antibody is NYRhEPO, and the immunogen is recombinant human erythropoietin .

Ig Subclass: The antibody belongs to the Mouse IgG1 subclass .

Purification Method: The antibody is purified using ion exchange chromatography .

Formulation and Storage: The antibody is typically supplied in a lyophilized form and should be reconstituted with sterile water before use. For long-term storage, it is recommended to store the lyophilized antibody at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C .

Applications

The Erythropoietin Mouse Anti Human (NYRhEPO) antibody is widely used in various research applications, including:

  • Direct ELISA: Enzyme-linked immunosorbent assay (ELISA) is a common technique used to detect and quantify specific proteins in a sample. The NYRhEPO antibody can be used in direct ELISA to measure erythropoietin levels .
  • Western Blot: This technique is used to detect specific proteins in a sample based on their size and reactivity with the antibody. The NYRhEPO antibody can be used to identify erythropoietin in Western blot assays .
  • Immunoprecipitation: This method is used to isolate and concentrate a specific protein from a complex mixture using an antibody. The NYRhEPO antibody can be used for immunoprecipitation of erythropoietin .
Safety and Usage

ProSpec’s products, including the Erythropoietin Mouse Anti Human (NYRhEPO) antibody, are furnished for laboratory research use only. They are not intended for use as drugs, agricultural or pesticidal products, food additives, or household chemicals .

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