EPHA2 Human, sf9
Description
EPHA2 is fused to 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
EPHA2 is a glycosylated protein that consists of an extracellular region, a single transmembrane segment, and a cytoplasmic tyrosine kinase domain. The extracellular region contains a ligand-binding domain, a cysteine-rich domain, and two fibronectin type III repeats. The cytoplasmic region includes a juxtamembrane segment, a tyrosine kinase domain, and a sterile alpha motif (SAM) domain.
The recombinant human EPHA2 protein expressed in Sf9 cells (a cell line derived from the fall armyworm, Spodoptera frugiperda) is produced using a baculovirus expression system. This system is advantageous for producing high yields of recombinant proteins with post-translational modifications similar to those in mammalian cells .
EPHA2 interacts with ephrin-A family ligands, which are membrane-bound proteins on adjacent cells. This interaction triggers bidirectional signaling: forward signaling through the Eph receptor and reverse signaling through the ephrin ligand. These signaling pathways regulate various cellular processes:
- Cell Migration and Adhesion: EPHA2 activation influences integrin-mediated adhesion and cell migration, which are critical for tissue development and repair.
- Cell Proliferation and Differentiation: EPHA2 signaling can either promote or inhibit cell proliferation and differentiation, depending on the cellular context.
- Tumor Growth and Metastasis: In cancer, EPHA2 is often overexpressed and associated with increased tumor growth, angiogenesis, and metastasis. It can also contribute to resistance to certain therapies .
Recombinant EPHA2 protein is widely used in research to study its role in various biological processes and diseases. It is particularly valuable in:
- Cancer Research: Understanding the mechanisms by which EPHA2 contributes to tumor progression and metastasis can lead to the development of targeted therapies.
- Drug Screening: Recombinant EPHA2 is used in high-throughput screening assays to identify potential inhibitors that can block its activity.
- Structural Studies: Analyzing the structure of EPHA2 helps in understanding its interaction with ligands and other proteins, which is crucial for designing effective drugs .
