Eotaxin 2 Rat
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
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Description
The CCL24 is purified by proprietary chromatographic techniques.
Product Specs
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Product Science Overview
Eotaxin-2, also known as Chemokine (C-C motif) ligand 24 (CCL24), is a member of the CC chemokine subfamily. It is a secreted protein encoded by the CCL24 gene and is produced by activated monocytes and T lymphocytes . Eotaxin-2 is known for its role in selectively attracting cells expressing the CCR3 receptor, including eosinophils, basophils, Th2 T cells, mast cells, and certain subsets of dendritic cells . This chemokine is involved in various physiological and pathological processes, including inflammation, immune response, and tumorigenesis .
Recombinant Rat Eotaxin-2/CCL24 is typically produced using Escherichia coli (E. coli) as the expression system . The recombinant protein is a single non-glycosylated polypeptide chain consisting of an N-terminal methionine and the mature rat Eotaxin-2 . The preparation process involves several steps:
- Expression: The gene encoding Eotaxin-2 is cloned into an expression vector and introduced into E. coli cells.
- Purification: The expressed protein is purified using techniques such as High-Performance Liquid Chromatography (HPLC) and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) to ensure high purity (>95%) .
- Lyophilization: The purified protein is lyophilized from a 0.2 μm filtered concentrated solution in phosphate-buffered saline (PBS), pH 7.4 .
The lyophilized protein is stable at 2-8°C and should be stored desiccated at -20°C for long-term storage . Upon reconstitution, the preparation is stable for up to one week at 2-8°C .
Eotaxin-2/CCL24 exhibits chemotactic activity, meaning it can induce the directed migration of cells . It selectively attracts cells expressing the CCR3 receptor, which includes eosinophils, basophils, Th2 T cells, mast cells, and certain subsets of dendritic cells . Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells .
In the context of tumorigenesis, CCL24 has been shown to contribute to cancer growth, metastasis, and host-tumor interactions . It is expressed in some tumor cells, such as colon cancer, hepatocellular carcinoma, and cutaneous T cell lymphoma . CCL24 can be used as a potential biomarker in several cancers, including colon cancer, non-small cell cancer, and nasopharyngeal carcinoma .
