Eotaxin 2 Human

CCL24 was initially cloned from a library of activated macrophages. It was shown that CCL24 binds to the receptor of eotaxin-1 (CCL11) and MCP-4 (CCL13). Additionally, CCL24 shares the CCR3 receptor with CCL26, CCL5, and CCL7 .

The human CCL24 protein consists of 93 amino acids with a predicted molecular mass of approximately 10.4 kDa. When expressed in E. coli, the DTT-reduced protein migrates at approximately 12 kDa and the non-reduced protein migrates at 14 kDa by SDS-PAGE . CCL24 is constitutively expressed in the jejunum and spleen and can be induced in the lung by allergen challenge and IL-4 .

CCL24 is known for its chemotactic properties, particularly for eosinophils, basophils, Th2 T cells, mast cells, and certain subsets of dendritic cells . It has lower chemotactic activity for neutrophils and none for monocytes and activated lymphocytes . CCL24 is a strong suppressor of colony formation by multipotential hematopoietic progenitor cell lines .

The eotaxin/CCR3 axis, which includes CCL24, has been extensively studied in the pathogenesis of asthma and allergy. It has also been associated with additional inflammatory and autoimmune disorders, including inflammatory bowel disease, multiple sclerosis, eosinophilic esophagitis, and rheumatoid arthritis . Furthermore, CCL24 has been linked to neovascular age-related macular degeneration .

Recombinant human CCL24 is produced in various expression systems, including E. coli and HEK293 cells. The recombinant protein is often purified to high levels of purity (>95%) and is used in research to study its biological functions and potential therapeutic applications .

Recombinant CCL24 can be stored at -20°C for up to six months or at -70°C or colder until the expiration date. For maximum results, it is recommended to quick spin the vial prior to opening and avoid repeated freeze/thaw cycles .