Eotaxin 2 Mouse

Eotaxin-2 Mouse Recombinant (CCL24)
Cat. No.
BT11235
Source
Escherichia Coli.
Synonyms
C-C motif chemokine 24, Small-inducible cytokine A24, Myeloid progenitor inhibitory factor 2, CK-beta-6, Eosinophil chemotactic protein 2, Eotaxin-2, CCL24, Ckb-6, MPIF2, MPIF-2, SCYA24, Eotaxin2, CCL-24.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity

Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

CCL24 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 93 amino acids and having a molecular mass of 10.3 kDa.
The CCL24 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Eotaxin-2, also known as MPIF2 and Ckb6, is a CC chemokine primarily produced by activated monocytes and T lymphocytes. It exhibits selective chemoattractant properties towards cells expressing the CCR3 receptor, including eosinophils, basophils, Th2 T cells, mast cells, and specific subsets of dendritic cells. Moreover, Eotaxin-2 demonstrates an inhibitory effect on the proliferation of multipotential hematopoietic progenitor cells. The mature form of this protein, characterized by C-terminal truncation, comprises 78 amino acids in humans and 92 amino acids in mice (without C-terminal truncation). Functionally, CCL24 acts as a chemotactic chemokine, attracting resting T lymphocytes and eosinophils. It exhibits weaker chemotactic activity towards neutrophils while showing no effect on monocytes and activated lymphocytes. Furthermore, CCL24 effectively suppresses colony formation in a multipotential hematopoietic progenitor cell line and binds to the CCR3 receptor.
Description
Recombinant CCL24 (Mouse) produced in E. coli is a single, non-glycosylated polypeptide chain composed of 93 amino acids. With a molecular weight of 10.3 kDa, it is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The CCL24 protein was lyophilized from a sterile solution at a concentration of 1 mg/ml, containing 20 mM phosphate buffer (pH 7.4) and 0.15 M sodium chloride.
Solubility
To reconstitute the lyophilized CCL24 (Mouse), it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Eotaxin-2, though stable at room temperature for up to 3 weeks, should ideally be stored in a dry environment below -18°C. Once reconstituted, CCL24 should be stored at 4°C for no more than 2-7 days. For extended storage, it is advisable to store it below -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity exceeding 97.0% as determined by: (a) Analysis using RP-HPLC. (b) Analysis using SDS-PAGE.
Biological Activity
The biological activity is evaluated based on its chemoattractant effect on murine lymphocytes. Employing a concentration range of 10-100 ng/ml, a Specific Activity of 10,000-100,000 IU/mg is observed.
Synonyms
C-C motif chemokine 24, Small-inducible cytokine A24, Myeloid progenitor inhibitory factor 2, CK-beta-6, Eosinophil chemotactic protein 2, Eotaxin-2, CCL24, Ckb-6, MPIF2, MPIF-2, SCYA24, Eotaxin2, CCL-24.
Source
Escherichia Coli.
Amino Acid Sequence
VTIPSSCCTS FISKKIPENR VVSYQLANGS ICPKAGVIFI TKKGHKICTD PKLLWVQRHI QKLDAKKNQP SKGAKAVRTK FAVQRRRGNS TEV.

Product Science Overview

Structure and Expression

Eotaxin-2 is produced by activated monocytes and T lymphocytes . The recombinant mouse Eotaxin-2 protein contains 93 amino acids and has a molecular weight of approximately 10.3 kDa . It is expressed in various tissues, including the jejunum and spleen, and can be induced in the lung by allergen challenge and interleukin-4 (IL-4) .

Function

Eotaxin-2 selectively chemoattracts cells expressing the CCR3 receptor. These cells include eosinophils, basophils, Th2 T cells, mast cells, and certain subsets of dendritic cells . It displays chemotactic activity on resting T lymphocytes and has minimal activity on neutrophils . Additionally, Eotaxin-2 is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line .

Applications

Recombinant mouse Eotaxin-2 is used in various research applications, including:

  • Western Blot (WB)
  • Immunohistochemistry (IHC)
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Functional Assays (FN)
  • Control Experiments (Ctrl)
Storage and Handling

The lyophilized preparation of recombinant mouse Eotaxin-2 is stable at 2°C to 8°C but should be kept at -20°C for long-term storage, preferably desiccated . Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C. For maximal stability, it is recommended to apportion the reconstituted preparation into working aliquots and store at -20°C, avoiding repeated freeze/thaw cycles .

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