ENPP1 Human
Description
The Human ENPP1 is purified by proprietary chromatographic techniques.
Product Specs
Provides background information on Ectonucleotide Pyrophosphatase (ENPP1), its characteristics, and its potential clinical significance.
Describes the product's appearance after the freeze-drying process.
Details the solution composition and concentration from which the ENPP1 protein is freeze-dried.
Provides recommendations for reconstituting the lyophilized ENPP1 protein and emphasizes the need for sterilization before cell culture use.
Offers storage recommendations for both the lyophilized and reconstituted forms of ENPP1 protein to maintain its stability.
Indicates the purity level of the ENPP1 protein product, determined by SDS-PAGE analysis.
Product Science Overview
ENPP1 is composed of two identical disulfide-bonded subunits. It has broad substrate specificity and is capable of cleaving phosphodiester bonds of nucleotides and nucleotide sugars, as well as pyrophosphate bonds of nucleotides and nucleotide sugars . The enzyme hydrolyzes nucleoside 5’-triphosphates to their corresponding monophosphates and can also hydrolyze diadenosine polyphosphates .
The recombinant form of human ENPP1 is produced in HEK cells and consists of a single, glycosylated polypeptide chain containing 840 amino acids. It has a calculated molecular mass of 96.5 kDa, although it migrates at approximately 110 kDa on SDS-PAGE due to glycosylation .
ENPP1 is involved in several critical biological processes, including bone mineralization and soft tissue calcification. It regulates pyrophosphate levels, which are essential for these processes . Additionally, ENPP1 is implicated in the metabolism of water-soluble vitamins and cofactors, as well as endochondral ossification .
Mutations in the ENPP1 gene have been associated with various medical conditions. These include idiopathic infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance . Diseases such as Cole Disease and Hypophosphatemic Rickets, Autosomal Recessive, 2 are also linked to ENPP1 .
