ENPP1 Mouse
Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by SDS-PAGE.
Description
ENPP1 Mouse Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain containing 828 amino acids (Lys85-Glu906) and having a molecular mass of 95.2kDa.
ENPP1 Mouse is fused to a 6 a.a his tag at C-Terminus and is purified by proprietary chromatographic techniques.
Product Specs
KEVKSCKGRC FERTFSNCRC DAACVSLGNC CLDFQETCVE PTHIWTCNKF RCGEKRLSRF VCSCADDCKT HNDCCINYSS VCQDKKSWVE ETCESIDTPE CPAEFESPPT LLFSLDGFRA EYLHTWGGLL PVISKLKNCG TYTKNMRPMY PTKTFPNHYS IVTGLYPESH GIIDNKMYDP KMNASFSLKS KEKFNPLWYK GQPIWVTANH QEVKSGTYFW PGSDVEIDGI LPDIYKVYNG SVPFEERILA VLEWLQLPSH ERPHFYTLYL EEPDSSGHSH GPVSSEVIKA LQKVDRLVGM LMDGLKDLGL DKCLNLILIS DHGMEQGSCK KYVYLNKYLG DVNNVKVVYG PAARLRPTDV PETYYSFNYE ALAKNLSCRE PNQHFRPYLK PFLPKRLHFA KSDRIEPLTF YLDPQWQLAL NPSERKYCGS GFHGSDNLFS NMQALFIGYG PAFKHGAEVD SFENIEVYNL MCDLLGLIPA PNNGSHGSLN HLLKKPIYNP SHPKEEGFLS QCPIKSTSND LGCTCDPWIV PIKDFEKQLN LTTEDVDDIY HMTVPYGRPR ILLKQHHVCL LQQQQFLTGY SLDLLMPLWA SYTFLRNDQF SRDDFSNCLY QDLRIPLSPV HKCSYYKSNS KLSYGFLTPP RLNRVSNHIY SEALLTSNIV PMYQSFQVIW HYLHDTLLQR YAHERNGINV VSGPVFDFDY DGRYDSLEIL KQNSRVIRSQ EILIPTHFFI VLTSCKQLSE TPLECSALES SAYILPHRPD NIESCTHGKR ESSWVEELLT LHRARVTDVE LITGLSFYQD RQESVSELLR LKTHLPIFSQ EDHHHHHH.
Product Science Overview
The ENPP family consists of several members, each with distinct substrate specificities and biological functions. These enzymes are typically membrane-bound glycoproteins that function at an alkaline pH. They hydrolyze a wide range of substrates, including nucleotides, lysophospholipids, and choline phosphate esters .
ENPP-6 is a specific member of the ENPP family that exhibits a high affinity for choline-containing substrates. It is a choline-specific glycerophosphodiester phosphodiesterase, meaning it primarily hydrolyzes choline phosphate esters . This enzyme is involved in the metabolism of choline, which is an essential nutrient for various physiological processes, including cell membrane integrity and neurotransmission.
Recombinant mouse ENPP-6 is a laboratory-produced version of the enzyme, derived from mouse cells. It is commonly used in research to study the enzyme’s structure, function, and potential therapeutic applications. The recombinant form is produced using human embryonic kidney (HEK293) cells, which are genetically engineered to express the mouse ENPP-6 protein .
The recombinant mouse ENPP-6 protein is typically tagged with a histidine (His) tag at the C-terminus to facilitate purification and detection. The protein has a predicted molecular mass of approximately 47 kDa, but it may appear as a band between 50-64 kDa on SDS-PAGE under reducing conditions due to glycosylation .
The enzyme’s activity is measured by its ability to cleave O-(4-Nitrophenylphosphoryl) choline, with a specific activity greater than 3,000 pmol/min/μg under the described conditions . This high specific activity indicates the enzyme’s efficiency in catalyzing the hydrolysis of its substrate.
ENPP-6 plays a vital role in the metabolism of choline-containing compounds, which are crucial for maintaining cell membrane structure and function. Choline is also a precursor for the neurotransmitter acetylcholine, which is essential for muscle function, memory, and other neurological processes. By regulating the levels of choline and its derivatives, ENPP-6 contributes to various physiological functions and overall cellular health.
Recombinant mouse ENPP-6 is widely used in biochemical and pharmacological research to investigate the enzyme’s role in health and disease. Understanding the enzyme’s structure and function can provide insights into its potential therapeutic applications, such as targeting ENPP-6 in diseases related to choline metabolism or developing inhibitors to modulate its activity.
