Introduction
Dual specificity phosphatase 21 (DUSP21) belongs to the protein-tyrosine phosphatase family. DUSPs deactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. DUSP21 can dephosphorylate single and diphosphorylated synthetic MAPK peptides, showing a preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. DUSP21 expression is restricted to the testis, where dephosphorylated phosphotyrosine residues are present in MAPK peptides.
Description
Recombinant human DUSP21, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 214 amino acids (residues 1-190) and possessing a molecular weight of 24.1 kDa. This protein includes a 24 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A sterile, colorless solution that has been filtered.
Formulation
The DUSP21 solution is provided at a concentration of 0.25 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 200 mM NaCl, 2 mM DTT, and 20% glycerol.
Stability
For optimal storage, refrigerate the product at 4°C if the entire vial will be consumed within 2-4 weeks. For extended storage, freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. It is crucial to minimize repeated freeze-thaw cycles.
Purity
The purity of this product exceeds 90%, as determined by SDS-PAGE analysis.
Synonyms
Dual specificity phosphatase 21, Low molecular weight dual specificity phosphatase 21, LMW-DSP21, BJ-HCC-26 tumor antigen, EC 3.1.3.16, EC 3.1.3.48.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMTASAS SFSSSQGVQQ PSIYSFSQIT RSLFLSNGVA ANDKLLLSSN RITAIVNASV EVVNVFFEGI QYIKVPVTDA RDSRLYDFFD PIADLIHTID MRQGRTLLHC MAGVSRSASL CLAYLMKYHS MSLLDAHTWT KSRRPIIRPN NGFWEQLINY EFKLFNNNTV RMINSPVGNI PDIYEKDLRM MISM