DUSP13 Human
Description
DUSP13 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The DUSP13 gene is located on chromosome 10q22.2 and encodes two distinct isoforms through the use of alternative, nonoverlapping open reading frames (ORFs): Testis- and Skeletal Muscle-Specific DSP (TMDP) and Muscle-Restricted DSP (MDSP) . These isoforms share 42% amino acid identity with each other and exhibit high similarity to their mouse counterparts .
DUSP13 is predominantly expressed in skeletal muscle and testis . The TMDP isoform is specifically expressed in testicular germ cells, particularly in spermatocytes and round spermatids, indicating its role in meiosis and differentiation during spermatogenesis . On the other hand, the MDSP isoform is expressed exclusively in skeletal muscle .
Recombinant human DUSP13 has been shown to possess intrinsic phosphatase activity, effectively dephosphorylating synthetic and protein substrates containing either phosphotyrosine or phosphothreonine residues . This activity underscores its importance in cellular signaling and regulation.
Recombinant human DUSP13 is typically produced in Escherichia coli as a single polypeptide chain containing 222 amino acids, with a molecular mass of approximately 24.7 kDa . The recombinant protein is often fused to a His-tag at the N-terminus to facilitate purification using chromatographic techniques .
Recombinant DUSP13 is utilized in various research applications, including studies on signal transduction pathways, cell cycle regulation, and apoptosis. Its ability to dephosphorylate both tyrosine and serine/threonine residues makes it a valuable tool for investigating the intricate mechanisms of cellular regulation.
