DUSP19 Human

Dual Specificity Phosphatase 19 Human Recombinant
Cat. No.
BT27766
Source
Escherichia Coli.
Synonyms
Dual specificity protein phosphatase 19, Dual specificity phosphatase TS-DSP1, Low molecular weight dual specificity phosphatase 3, LMW-DSP3, Protein phosphatase, SKRP1, Stress-activated protein kinase pathway-regulating phosphatase 1, SAPK pathway-regulating phosphatase 1, DUSP19, DUSP17, LMWDSP3, TS-DSP1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DUSP19 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 176 amino acids (65-217) and having a molecular mass of 19.4kDa.
DUSP19 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Dual Specificity Phosphatases (DUSPs) are a group of enzymes known for their ability to remove phosphate groups from both tyrosine and serine/threonine residues within proteins. This unique characteristic distinguishes them within the larger family of phosphatases. DUSPs play a crucial role in regulating critical signaling pathways within cells, acting as key modulators of cellular responses. DUSP19, a specific member of the DUSP subfamily, acts as a phosphatase that specifically targets and regulates stress-activated protein kinase pathways. Unlike some DUSPs, it lacks the N-terminal CH2 domain characteristic of the MKP class. Notably, DUSP19 possesses a distinct variation in its C-terminal catalytic domain, with the terminal serine residue being substituted with an alanine.
Description
Recombinant human DUSP19, produced in E. coli, is a single polypeptide chain that lacks glycosylation. This protein consists of 176 amino acids (residues 65-217), resulting in a molecular weight of 19.4 kDa. For purification purposes, a 23 amino acid His-tag is attached to the N-terminus, enabling efficient purification via proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The DUSP19 solution is provided at a concentration of 0.5 mg/ml and is formulated in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, 30% glycerol, and 0.1M NaCl.
Stability
For short-term storage (up to 2-4 weeks), the DUSP19 solution can be kept at 4°C. For extended storage, freezing at -20°C is recommended. To ensure optimal stability during long-term storage, consider adding a carrier protein such as HSA or BSA to a final concentration of 0.1%. Repeated freezing and thawing of the solution should be avoided to maintain protein integrity.
Purity
The purity of the DUSP19 protein is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Dual specificity protein phosphatase 19, Dual specificity phosphatase TS-DSP1, Low molecular weight dual specificity phosphatase 3, LMW-DSP3, Protein phosphatase, SKRP1, Stress-activated protein kinase pathway-regulating phosphatase 1, SAPK pathway-regulating phosphatase 1, DUSP19, DUSP17, LMWDSP3, TS-DSP1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSQVGVIKP WLLLGSQDAA HDLDTLKKNK VTHILNVAYG VENAFLSDFT YKSISILDLP ETNILSYFPE CFEFIEEAKR KDGVVLVHCN AGVSRAAAIV IGFLMNSEQT SFTSAFSLVK NARPSICPNS GFMEQLRTYQ EGKESNKCDR IQENSS.

Product Science Overview

Introduction

Dual Specificity Phosphatase 19 (DUSP19) is a member of the dual specificity protein phosphatase subfamily. These phosphatases are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. DUSP19 is also known by several other names, including TS-DSP1, LMW-DSP3, and SKRP1. This enzyme plays a crucial role in regulating various cellular processes by modulating critical signaling pathways .

Structure and Function

DUSP19 is a protein phosphatase that functions as a stress-activated protein kinase pathway-regulating phosphatase. It is involved in the dephosphorylation of specific substrates, thereby modulating their activity and function. The recombinant form of DUSP19 is typically produced in E. coli and includes an N-terminal His-tag for purification purposes .

Biological Significance

DUSP19 has been implicated in various cellular processes, including cell growth, differentiation, and apoptosis. It is particularly important in the regulation of the stress-activated protein kinase (SAPK) pathway, which is involved in the cellular response to stress and inflammation . Additionally, DUSP19 has been shown to interact with vascular endothelial growth factor receptor 3 (VEGFR3), playing a role in the regulation of cancer cell invasiveness .

Applications

Recombinant DUSP19 is widely used in research to study its role in cellular signaling pathways and its potential as a therapeutic target. It is also utilized in various biochemical assays to investigate its substrate specificity and enzymatic activity .

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