DPPA5 Human

Developmental Pluripotency Associated 5 Human Recombinant
Cat. No.
BT30101
Source
Escherichia Coli.
Synonyms
ESG1, Developmental pluripotency-associated 5 proteins, hDPPA5, Embryonal stem cell-specific gene 1 protein, ESG-1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

DPPA5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 139 amino acids (1-116 a.a) and having a molecular mass of 15.9kDa.
DPPA5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Developmental pluripotency-associated 5 protein (DPPA5) is a protein composed of 116 amino acids. It is found in the cytoplasm and possesses a single KH domain. DPPA5 is present in embryonic germ (EG) cells, primordial germ (PG) cells, and embryonic stem (ES) cells. It plays a crucial role in maintaining the pluripotency of ES cells and may be vital for normal embryo development.
Description
Recombinant human DPPA5, produced in E. coli, is a single polypeptide chain that lacks glycosylation. It consists of 139 amino acids, encompassing amino acids 1 to 116, and has a molecular weight of 15.9 kDa. A 23 amino acid His-tag is fused to the N-terminus of DPPA5. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The DPPA5 protein solution has a concentration of 0.25 mg/ml and contains 20mM Phosphate buffer with a pH of 8.0 and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeatedly freezing and thawing the product.
Purity
SDS-PAGE analysis has determined the purity to be greater than 85.0%.
Synonyms
ESG1, Developmental pluripotency-associated 5 proteins, hDPPA5, Embryonal stem cell-specific gene 1 protein, ESG-1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGTLPAR RHIPPWVKVP EDLKDPEVFQ VQTRLLKAIF GPDGSRIPYIEQVSKAMLEL KALESSDLTE VVVYGSYLYK LRTKWMLQSM AEWHRQRQER GMLKLAEAMN ALELGPWMK.

Product Science Overview

Gene and Protein Structure

The DPPA5 gene belongs to the KHDC1/DPPA5/ECAT1/OOEP gene family, which encodes structurally related proteins characterized by an atypical RNA-binding K Homology (KH) domain . This domain is essential for the protein’s function in binding to specific target mRNAs . The gene is located on chromosome 6 in humans and has several pseudogenes on other chromosomes .

Function and Mechanism

DPPA5 is primarily involved in the maintenance of pluripotency in embryonic stem cells. It is dispensable for the self-renewal of pluripotent ESCs and the establishment of germ cells . The protein associates with specific target mRNAs, which is crucial for its role in maintaining pluripotency .

One of the key functions of DPPA5 is its interaction with the NANOG protein, a critical factor in maintaining the pluripotent state of stem cells. DPPA5 stabilizes NANOG and supports human pluripotent stem cell (hPSC) self-renewal and cell reprogramming in feeder-free conditions . This interaction enhances the function of NANOG, thereby promoting the pluripotent state of the cells .

Expression and Regulation

The expression of DPPA5 is a specific marker for pluripotent stem cells. It is highly expressed in human pluripotent stem cells (hPSCs) grown on feeder-free substrates such as Matrigel, Laminin-511, and Vitronectin . Overexpression of DPPA5 in hPSCs increases NANOG protein levels via a post-transcriptional mechanism .

Applications in Regenerative Medicine

Understanding the function and regulation of DPPA5 is crucial for advancements in regenerative medicine. The ability of DPPA5 to enhance the reprogramming efficiency of human somatic cells to induced pluripotent stem cells (hiPSCs) makes it a valuable tool in developing therapies for various diseases . Improved protocols in hPSC maintenance and reprogramming, supported by DPPA5, may contribute to the future application of these cells in regenerative medicine .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

DPPA3 Human
Developmental Pluripotency Associated 3 Human Recombinant
Cat. No.
BT29979
Source
Escherichia Coli.
DPPA4 Human
Developmental Pluripotency Associated 4 Human Recombinant
Cat. No.
BT30020
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.