DPH2 Human
Sf9, Baculovirus cells.
DPH2 Homolog, S-Adenosyl-L-Methionine:L-Histidine 3-Amino-3-Carboxypropyltransferase 2, Diphtheria Toxin Resistance Protein 2, Diphthamide Biosynthesis Protein 2, DPH2L2, Diptheria Toxin Resistance Protein Required For Diphthamide Biosynthesis-Like 2, 2-(3-Amino-3-Carboxypropyl)Histidine Synthase Subunit 2, Diphthamide Biosynthesis Protein 2 Homolog-Like 2, Diphthamide Biosynthesis-Like Protein 2, EC 2.5.1.108, DPH2-Like 2, DPH2.
Greater than 85.0% as determined by SDS-PAGE.
Description
DPH2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 497 amino acids (1-489a.a.) and having a molecular mass of 53.1kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). DPH2 is expressed with an 8 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
DPH2 Homolog, S-Adenosyl-L-Methionine:L-Histidine 3-Amino-3-Carboxypropyltransferase 2, Diphtheria Toxin Resistance Protein 2, Diphthamide Biosynthesis Protein 2, DPH2L2, Diptheria Toxin Resistance Protein Required For Diphthamide Biosynthesis-Like 2, 2-(3-Amino-3-Carboxypropyl)Histidine Synthase Subunit 2, Diphthamide Biosynthesis Protein 2 Homolog-Like 2, Diphthamide Biosynthesis-Like Protein 2, EC 2.5.1.108, DPH2-Like 2, DPH2.
Sf9, Baculovirus cells.
MESMFSSPAE AALQRETGVP GLLTPLPDLD GVYELERVAG FVRDLGCERV ALQFPDQLLG DAVAVAARLE ETTGSKMFIL GDTAYGSCCV DVLGAEQAGA QALIHFGPAC LSPPARPLPV AFVLRQRSVA LELCVKAFEA QNPDPKAPVV LLSEPACAHA LEALATLLRP RYLDLLVSSP AFPQPVGSLS PEPMPLERFG RRFPLAPGRR LEEYGAFYVG GSKASPDPDL DPDLSRLLLG WAPGQPFSSC CPDTGKTQDE GARAGRLRAR RRYLVERARD ARVVGLLAGT LGVAQHREAL AHLRNLTQAA GKRSYVLALG RPTPAKLANF PEVDVFVLLA CPLGALAPQL SGSFFQPILA PCELEAACNP AWPPPGLAPH LTHYADLLPG SPFHVALPPP ESELWETPDV SLITGDLRPP PAWKSSNDHG SLALTPRPQL ELAESSPAAS FLSSRSWQGL EPRLGQTPVT EAVSGRRGIA IAYEDEGSGL EHHHHHH.
Product Science Overview
Diphthamide is a unique post-translationally modified histidine residue found in elongation factor 2 (eEF2), which is essential for protein synthesis in eukaryotic cells. The biosynthesis of diphthamide involves a multi-step enzymatic process, and the Diphthamide Biosynthesis 2 (DPH2) protein plays a crucial role in this pathway. This article delves into the background, function, and significance of DPH2, particularly focusing on its human recombinant form.
The biosynthesis of diphthamide is a complex process that requires the coordinated action of several proteins, including DPH1, DPH2, DPH3, DPH4, DPH5, DPH6, and DPH7. The pathway can be divided into three main steps:
- Initial Modification: The first step involves the transfer of a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a specific histidine residue on eEF2. This reaction is catalyzed by the DPH1-DPH2 complex .
- Intermediate Formation: The second step involves further modifications to form an intermediate compound. This step is facilitated by DPH3 and a NADH-dependent reductase .
- Amidation: The final step is the amidation of the intermediate to form diphthamide. The enzymes involved in this step are not fully characterized .
DPH2 is a key enzyme in the first step of diphthamide biosynthesis. It works in conjunction with DPH1 to transfer the ACP group from SAM to the histidine residue on eEF2. This modification is critical for the proper functioning of eEF2, which is responsible for the translocation of the ribosome along mRNA during protein synthesis .
Mutations in the DPH2 gene can lead to diphthamide deficiency syndrome, a rare autosomal-recessive disorder characterized by intellectual disability, developmental abnormalities, and other morphological features. The syndrome results from reduced activity of diphthamide-synthesizing enzymes, which compromises the placement of diphthamide on eEF2 and affects translation accuracy .
Recombinant DPH2 refers to the DPH2 protein that is produced using recombinant DNA technology. This involves inserting the DPH2 gene into an expression system, such as bacteria or yeast, to produce the protein in large quantities. Recombinant DPH2 is used in research to study the diphthamide biosynthesis pathway and to develop potential therapeutic interventions for diphthamide deficiency syndrome .
