Dengue NS1 Antibody
Monoclonal Mouse Anti Dengue NS1
Cat. No.
BT5970
Source
Synonyms
Appearance
2 vials of sterile Filtered clear colorless solution.
Purity
95% pure (12% SDS-PAGE and Coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Paired monoclonal antibodies developed for dengue NS1 antigen test by lateral flow immunoassay device. First antibody is for conjugation and the second antibody is for membrane coating.
Please note that when ordering for example: 100µg antibody we ship 50µg from each of the antibody solutions (100µg in total).
Please note that when ordering for example: 100µg antibody we ship 50µg from each of the antibody solutions (100µg in total).
Product Specs
Description
This product consists of two monoclonal antibodies designed for use in a dengue NS1 antigen lateral flow immunoassay. The first antibody is intended for conjugation, while the second antibody is meant for membrane coating. When ordering, for instance, 100µg of antibody, you will receive 50µg of each antibody solution (totaling 100µg).
Purity
The purity level of this antibody is 95%, as determined by SDS-PAGE and Coomassie staining.
Physical Appearance
This product is supplied as two vials containing a clear, colorless solution that has been sterile filtered.
Formulation
* One vial contains a 3.6 mg/ml solution of Dengue NS1 Antibody in PBS and Sodium nitrate, specifically formulated for gold conjugation.
* The other vial contains a 2.2 mg/ml solution of Dengue NS1 Antibody in PBS and Sodium nitrate, intended for membrane coating.
Stability
For optimal storage, the antibody should be kept at 4°C if it will be used within 2-4 weeks. For longer storage periods, it is recommended to freeze the antibody at -20°C. To ensure long-term stability, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Assay Conditions
1. Utilize freshly prepared gold particles (1-2 days old) with optimal homogeneity in size (λ 525-529 nm, OD 1.2-1.4).
2. Perform conjugation at a pH of 8.5 for a duration of 6-8 hours.
3. Blocking conditions involve removing the supernatant and resuspending the gold pellet bound with anti-dengue NS1 to an OD of 8.0-8.2. Add casein to achieve a final concentration of 0.3-0.4%.
4. Treat the sample pad with a solution containing 50 mM sodium borate, 0.5% mouse serum, and 0.5% Triton X-100 at a pH of 8.0.
Applications
These antibodies are designed for use in lateral flow immunoassays.
Purification Method
Purified monoclonal IgG by protein A chromatography.
Product Science Overview
Introduction
Dengue fever is a mosquito-borne viral infection that has become a significant public health concern in tropical and subtropical regions. The disease is caused by the dengue virus (DENV), which has four distinct serotypes (DENV-1, DENV-2, DENV-3, and DENV-4). One of the critical components of the dengue virus is the non-structural protein 1 (NS1), a glycoprotein essential for viral replication, infection, and immune evasion . Monoclonal antibodies targeting NS1 have been developed to aid in the diagnosis and research of dengue infections.
Development of Monoclonal Mouse Anti-Dengue NS1
Monoclonal antibodies are produced by identical immune cells that are clones of a unique parent cell. The development of monoclonal mouse anti-Dengue NS1 involves several steps:
- Immunization: Mice are immunized with the NS1 protein derived from the dengue virus. This process stimulates the mouse’s immune system to produce antibodies against the NS1 protein.
- Hybridoma Technology: Spleen cells from the immunized mice, which produce antibodies, are fused with myeloma cells (cancer cells) to create hybrid cells known as hybridomas. These hybridomas can proliferate indefinitely and produce large quantities of the desired antibody.
- Screening and Selection: The hybridomas are screened to identify those producing antibodies that specifically bind to the NS1 protein. The selected hybridomas are then cloned to ensure the production of a monoclonal antibody.
- Purification: The monoclonal antibodies are purified from the hybridoma culture supernatant using techniques such as protein A or G affinity chromatography .
Applications of Monoclonal Mouse Anti-Dengue NS1
Monoclonal mouse anti-Dengue NS1 antibodies have several applications in research and diagnostics:
- Diagnostic Assays: These antibodies are used in various diagnostic assays, such as enzyme-linked immunosorbent assays (ELISA) and Western blotting, to detect the presence of NS1 protein in patient samples. This helps in the early diagnosis of dengue infection .
- Histopathological Analysis: Monoclonal antibodies targeting NS1 are used in immunohistochemical assays to detect dengue virus antigens in tissue samples. This is particularly useful in the differential diagnosis of hemorrhagic fevers and other infectious diseases .
- Research: These antibodies are valuable tools in studying the role of NS1 in dengue virus pathogenesis and immune response. They help researchers understand how the virus evades the immune system and causes disease .
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