Dengue Envelope-3, Insect
Description
Dengue Envelope-3 is purified by proprietary chromatographic technique.
Product Specs
Product Science Overview
Dengue virus (DENV) is a mosquito-borne virus that causes dengue fever, a significant public health concern in tropical and subtropical regions. There are four distinct serotypes of the dengue virus: DENV-1, DENV-2, DENV-3, and DENV-4. Among these, DENV-3 is known for causing severe outbreaks and is associated with severe disease manifestations such as dengue hemorrhagic fever and dengue shock syndrome.
Recombinant technology has been employed to develop vaccines and therapeutic proteins for dengue virus. Recombinant dengue virus subtype 3 (DENV-3) proteins are produced using various expression systems, including bacterial, yeast, insect, and mammalian cells. Among these, insect cells have gained popularity due to their ability to perform post-translational modifications similar to those in mammalian cells, which is crucial for the proper folding and functionality of the recombinant proteins.
The insect cell expression system utilizes cells derived from insects, such as Spodoptera frugiperda (Sf9) or Trichoplusia ni (High Five), to produce recombinant proteins. The baculovirus expression vector system (BEVS) is commonly used in this context. BEVS involves the insertion of the gene encoding the target protein into the baculovirus genome, which is then used to infect insect cells. The infected cells produce high yields of the recombinant protein.
The production of DENV-3 recombinant proteins in insect cells involves several steps:
- Gene Cloning: The gene encoding the desired DENV-3 protein, such as the envelope protein domain III (EDIII), is cloned into a baculovirus transfer vector.
- Transfection: The recombinant transfer vector is used to transfect insect cells, leading to the production of recombinant baculovirus.
- Infection: The recombinant baculovirus is used to infect a larger culture of insect cells.
- Protein Expression: Infected insect cells express the DENV-3 recombinant protein, which can be harvested from the cell culture supernatant or cell lysate.
- Purification: The recombinant protein is purified using techniques such as affinity chromatography, ensuring high purity and yield.
Recombinant DENV-3 proteins produced in insect cells have several applications:
- Vaccine Development: Recombinant proteins are used as antigens in subunit vaccines. These vaccines aim to elicit an immune response without causing disease.
- Diagnostic Tools: Recombinant proteins are used in diagnostic assays to detect antibodies against DENV-3 in patient samples.
- Research: Recombinant proteins are used in various research studies to understand the structure, function, and immunogenicity of DENV-3 proteins.
