Dengue Envelope-3 45kDa
Protein is >95% pure as determined by 10% SDS-PAGE (coomassie staining).
Description
Recombinant Dengue Virus Subtype 3 Envelope 45kDa (a.a 43-413) is produced in E.coli. This protein is fused to 6xHis tag at C-terminus.
Product Specs
Purified by proprietary chromatographic technique.
Product Science Overview
Dengue virus (DENV) is a mosquito-borne virus that poses a significant global health threat, particularly in tropical and subtropical regions. There are four distinct serotypes of the dengue virus (DENV-1, DENV-2, DENV-3, and DENV-4), each capable of causing dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. The envelope (E) protein of the dengue virus is a critical component for viral entry into host cells and is a major target for neutralizing antibodies.
DENV-3 is one of the four serotypes of the dengue virus. It has been associated with severe outbreaks and is known for its ability to cause severe disease manifestations. The envelope protein of DENV-3, particularly the domain III (EDIII), is of great interest for vaccine development and diagnostic purposes due to its role in eliciting a strong immune response.
The recombinant envelope protein of DENV-3, specifically the 45kDa recombinant, is a laboratory-produced version of the viral protein. This recombinant protein is used in research to study the immune response to DENV-3 and to develop potential vaccines and diagnostic tools. The production of recombinant proteins involves inserting the gene encoding the protein into a host organism, such as bacteria, yeast, or mammalian cells, which then produces the protein in large quantities.
The production of recombinant DENV-3 envelope protein has been optimized in various systems. For instance, the Pichia pastoris yeast system has been used to produce the envelope domain III (EDIII) of DENV-3 . This system allows for high-level expression and secretion of the protein, which is crucial for its use in research and vaccine development. Optimization of culture conditions, such as temperature and media composition, has been shown to significantly enhance the yield of the recombinant protein .
The recombinant DENV-3 envelope protein has several important applications:
- Vaccine Development: The EDIII of the envelope protein is a promising candidate for dengue vaccines. It can induce a strong immune response and generate neutralizing antibodies that protect against the virus .
- Diagnostic Tools: The recombinant protein can be used to develop diagnostic assays for detecting dengue virus infections. These assays can help in the early diagnosis and management of dengue fever .
- Research: The recombinant protein is used in various research studies to understand the structure and function of the dengue virus envelope protein, as well as the immune response it elicits .
