Dengue Envelope-2, Insect
Description
Recombinant Dengue Virus Subtype 2 produced in Insect Cells is a polypeptide chain containing amino acids 2-395 & having a molecular weight of approximately 45.3 kDa.
Dengue Envelope-2 is purified by proprietary chromatographic technique.
Product Specs
Product Science Overview
Dengue virus (DENV) is a significant global health concern, causing millions of infections annually. Among the four serotypes of DENV, Dengue Virus Subtype 2 (DENV-2) is particularly notable for its widespread transmission and severe disease manifestations. The development of recombinant DENV-2 using insect cells has emerged as a promising approach for studying the virus and developing vaccines.
DENV is a single-stranded, positive-sense RNA virus belonging to the genus Flavivirus within the family Flaviviridae . The virus is primarily transmitted to humans through the bite of infected Aedes mosquitoes, particularly Aedes aegypti . DENV infections can range from mild dengue fever to severe dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS), which can be fatal .
Recombinant DENV-2 refers to genetically engineered versions of the virus, created to study its properties or develop vaccines. These recombinant viruses are often produced using insect cell systems, such as the Aedes aegypti Aag2 cell line . The use of insect cells offers several advantages, including high yield production and the ability to perform post-translational modifications similar to those in the natural mosquito vector .
Insect cell systems, particularly those derived from Aedes mosquitoes, are widely used for the production of recombinant DENV-2. These systems provide a suitable environment for the virus to replicate and express its proteins. The Aedes aegypti Aag2 cell line is commonly used due to its susceptibility to DENV infection and its ability to produce high titers of the virus . Additionally, the C6/36 Aedes albopictus cell line is also utilized for similar purposes .
The development of recombinant DENV-2 using insect cells has several important applications:
- Vaccine Development: Recombinant DENV-2 can be used to create vaccine candidates that elicit strong immune responses. These vaccines can be tested for safety and efficacy in preclinical and clinical trials.
- Virus-Host Interaction Studies: By using recombinant DENV-2, researchers can study the interactions between the virus and its host cells, gaining insights into viral replication, immune evasion, and pathogenesis.
- Drug Screening: Recombinant DENV-2 can be employed in high-throughput screening assays to identify potential antiviral compounds that inhibit viral replication.
