Dengue Envelope-2 45kDa

Dengue Virus Subtype 2 Envelope 45kDa Recombinant
Cat. No.
BT2965
Source
E.coli.
Synonyms
Appearance
Sterile filtered colorless solution.
Purity
Protein is >95% pure as determined by 12% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant Dengue Virus Subtype 2 Envelope (a.a 43-413) migrates at 45kDa, produced in E.coli, this protein is fused to 6xHis tag at the C-terminus.

Product Specs

Introduction
Dengue fever is caused by one of four closely related virus serotypes belonging to the genus Flavivirus, family Flaviviridae. After a primary infection, individuals can be infected again by a different serotype. Detecting antibodies specific to dengue viruses is crucial for clinical diagnosis. Lateral flow rapid tests have emerged as a preferred and widely recognized method for this purpose. However, manufacturers face challenges in obtaining dengue antigens that offer complete coverage for both Dengue IgG and IgM recognition across all four serotypes while also possessing good colloidal gold binding properties. To address this, eight well-characterized dengue antigens have been developed for lateral flow products, ensuring comprehensive coverage for dengue IgG and IgM detection. Researchers can select the most suitable product from these options based on their specific application requirements.
Description
This recombinant protein consists of the Dengue Virus Subtype 2 Envelope domain (amino acids 43-413). Expressed in E.coli, it has a molecular weight of 45kDa and is fused to a 6xHis tag at its C-terminus.
Purity
Analysis by 12% SDS-PAGE followed by Coomassie staining indicates a purity greater than 95%.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The protein solution is buffered with Phosphate buffer and supplemented with 50mM arginine.
Stability
For optimal storage, Dengue Envelope-2 45kDa should be kept at -18°C. While it remains stable at 4°C for up to one week, repeated freezing and thawing should be avoided.
Source
E.coli.
Purification Method

Purified by proprietary chromatographic technique.

Product Science Overview

Introduction

Dengue fever is a mosquito-borne viral infection caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae. The four serotypes are sufficiently different that there is no cross-protection, and epidemics caused by multiple serotypes (hyperendemicity) can occur. The Dengue Virus Subtype 2 Envelope 45kDa Recombinant is a significant protein used in research and diagnostics related to dengue virus.

Structure and Production

The Dengue Virus Subtype 2 Envelope (a.a 43-413) migrates at 45kDa and is produced in E. coli. This protein is fused to a 6xHis tag at the C-terminus . The recombinant protein is purified using proprietary chromatographic techniques to ensure high purity, typically greater than 95% as determined by 12% PAGE (coomassie staining) .

Applications

The recombinant Dengue Virus Subtype 2 Envelope protein is widely used in various applications:

  • Immunization Protocols: It is ideal for generating antibodies that specifically target the protein of interest .
  • Clinical Diagnostics: Detection of particular antibodies to dengue viruses is used for diagnosis in clinical settings. The recombinant protein is suitable for gold conjugation and has been tested for strong Dengue IgG and IgM reactivity using lateral flow devices .
  • Research: It is used in laboratory research to study the virus’s structure, function, and interactions with host cells .
Stability and Storage

The recombinant protein is stable at 4°C for one week but should be stored below -18°C to prevent freeze-thaw cycles, which can degrade the protein .

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THE BIOTEK
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