DCN Human, Sf9
Decorin Human Recombinant, Sf9
Cat. No.
BT29242
Source
Sf9,
Baculovirus cells.
Synonyms
Decorin, Decorin Proteoglycan, Bone Proteoglycan II, SLRR1B,
PG-S2, CSCD, PG40,
Dermatan Sulphate Proteoglycans II, Small Leucine-Rich
Protein 1B, Proteoglycan Core Protein, DSPG2, PGII, PGS2, Decorin, Bone
proteoglycan II, PG-S2, , PG40.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
DCN produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 335 amino acids (31-359a.a.) and
having a molecular mass of 37.1kDa.
(Molecular size on SDS-PAGE will appear at
approximately 40-57kDa).
DCN is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Decorin (DCN) is a small proteoglycan found in the cellular and pericellular matrix. It shares structural similarities with the biglycan protein. As a secreted protein, Decorin exhibits binding affinity for collagen and fibronectin within the extracellular matrix. Notably, Decorin exists in various glycoforms, incorporating chondroitin sulfate or dermatan sulfate, reflecting its tissue of origin. Each DCN molecule possesses a single glycosaminoglycan chain attachment. Decorin plays a role in regulating the rate of fibril formation and has demonstrated the ability to inhibit the growth of several tumor cell lines. Genetic defects in the DCN gene have been linked to corneal dystrophy, and the gene itself is considered a candidate for Marfan syndrome.
Description
Produced in Sf9 Baculovirus cells, our recombinant DCN is a single, glycosylated polypeptide chain consisting of 335 amino acids (31-359 a.a.). It has a molecular mass of 37.1 kDa. Note: The apparent molecular size on SDS-PAGE is expected to be between 40-57 kDa. Our DCN is engineered with a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
The DCN protein solution is provided at a concentration of 1 mg/ml and is formulated in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure long-term stability during frozen storage, consider adding a carrier protein such as HSA or BSA (0.1%). It's crucial to avoid repeated freeze-thaw cycles.
Purity
The purity of DCN is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Decorin, Decorin Proteoglycan, Bone Proteoglycan II, SLRR1B,
PG-S2, CSCD, PG40,
Dermatan Sulphate Proteoglycans II, Small Leucine-Rich
Protein 1B, Proteoglycan Core Protein, DSPG2, PGII, PGS2, Decorin, Bone
proteoglycan II, PG-S2, , PG40.
Source
Sf9,
Baculovirus cells.
Amino Acid Sequence
DEASGIGPEV PDDRDFEPSL GPVCPFRCQC HLRVVQCSDL GLDKVPKDLP
PDTTLLDLQN NKITEIKDGD FKNLKNLHAL ILVNNKISKV SPGAFTPLVK LERLYLSKNQ LKELPEKMPK
TLQELRAHEN EITKVRKVTF NGLNQMIVIE LGTNPLKSSG IENGAFQGMK KLSYIRIADT
NITSIPQGLP PSLTELHLDG NKISRVDAAS LKGLNNLAKL GLSFNSISAV
DNGSLANTPH LRELHLDNNK LTRVPGGLAE HKYIQVVYLH NNNISVVGSS DFCPPGHNTK KASYSGVSLF
SNPVQYWEIQ PSTFRCVYVR SAIQLGNYKH HHHHH.
Product Science Overview
Structure and Composition
Decorin is composed of a core protein and a glycosaminoglycan (GAG) chain, which can be either chondroitin sulfate or dermatan sulfate . The core protein contains leucine-rich repeats (LRRs) that are essential for its interaction with other ECM components . The human decorin cDNA encodes a 359 amino acid precursor, which includes a 16 amino acid signal sequence and a 14 amino acid propeptide .
Biological Functions
Decorin is involved in various biological processes:
- Collagen Fibrillogenesis: Decorin modulates collagen fibrillogenesis, which is crucial for the formation and maintenance of the ECM .
- Cell Signaling: Decorin interacts with several cell surface receptors, including the epidermal growth factor receptor (EGFR) and other members of the ErbB family, thereby influencing cell growth and survival .
- Tumor Microenvironment: Decorin resides in the tumor microenvironment and affects the biology of various types of cancer by downregulating the activity of several receptors involved in cell growth and survival .
Recombinant Production in Sf9 Cells
Applications
Recombinant human decorin has several applications in research and medicine:
- Tissue Engineering: Due to its role in collagen fibrillogenesis, decorin is used in tissue engineering to promote the formation of functional ECM.
- Cancer Research: Decorin’s ability to modulate cell signaling pathways makes it a valuable tool in cancer research, particularly in studies focused on the tumor microenvironment .
- Wound Healing: Decorin is also explored for its potential in enhancing wound healing by promoting ECM formation and modulating inflammatory responses.
Storage and Stability
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