DCN Human
Decorin Human Recombinant
Cat. No.
BT29188
Source
Escherichia Coli.
Synonyms
Decorin, Bone proteoglycan II, PG-S2, PG40, DCN, SLRR1B, CSCD, PGII, PGS2, DSPG2.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
DCN Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 350 amino acids (31-359 a.a) and having a molecular mass of 38.6kDa.
DCN is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
DCN is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Decorin (DCN) is a small proteoglycan found in the cellular or pericellular matrix. It shares structural similarities with the biglycan protein. As a secreted protein, decorin binds to collagen and fibronectin within the extracellular matrix. Different glycoforms of decorin exist, characterized by the presence of chondroitin sulfate or dermatan sulfate, depending on the tissue of origin. Notably, DCN possesses a single attached glycosaminoglycan chain. Decorin plays a role in regulating the rate of fibril formation and has been shown to inhibit the growth of various tumor cell lines. Defects in the DCN gene are associated with corneal dystrophy. Furthermore, the DCN gene is considered a candidate gene for Marfan syndrome.
Description
Recombinant Human DCN, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 350 amino acids (31-359 a.a) and has a molecular mass of 38.6 kDa. The DCN protein is fused to a 21 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Colorless, sterile-filtered solution.
Formulation
The DCN protein solution has a concentration of 0.25 mg/ml and is supplied in a buffer containing 20 mM Tris (pH 8.0) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure long-term stability, consider adding a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing should be avoided.
Purity
The purity of the product is greater than 80%, as determined by SDS-PAGE analysis.
Synonyms
Decorin, Bone proteoglycan II, PG-S2, PG40, DCN, SLRR1B, CSCD, PGII, PGS2, DSPG2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MDEASGIGPE VPDDRDFEPS LGPVCPFRCQ CHLRVVQCSDLGLDKVPKDL PPDTTLLDLQ NNKITEIKDG DFKNLKNLHA LILVNNKISK VSPGAFTPLVKLERLYLSKN QLKELPEKMP KTLQELRAHE NEITKVRKVT FNGLNQMIVI ELGTNPLKSS GIENGAFQGM KKLSYIRIAD TNITSIPQGL PPSLTELHLD GNKISRVDAA SLKGLNNLAKLGLSFNSISA VDNGSLANTP HLRELHLDNN KLTRVPGGLA EHKYIQVVYL HNNNISVVGSSDFCPPGHNT KKASYSGVSL FSNPVQYWEI QPSTFRCVYV RSAIQLGNYK.
Product Science Overview
Structure and Composition
Decorin is characterized by its structure, which includes:
- N-terminal and C-terminal cysteine-rich regions: These regions flank the central part of the protein.
- Leucine-rich repeats (LRR): The central region contains 10-12 tandem LRRs .
The human Decorin cDNA encodes a 359 amino acid precursor, which includes a 16 amino acid signal sequence and a 14 amino acid propeptide . The mature form of Decorin contains twelve tandem LRRs and is N-glycosylated. It also carries a variably-sized hybrid chondroitin/dermatan sulfate chain at Ser34 .
Function and Biological Activity
Decorin is primarily involved in:
- Regulating collagen fibrillogenesis: It modulates the formation and organization of collagen fibers in the ECM .
- Interacting with growth factors: Decorin regulates the bioactivity of matrix-associated growth factors such as FGF-2, GDF-8/Myostatin, TGF-beta, and WISP-1 .
- Binding and activating receptors: It binds to and activates receptors like EGF R, ErbB4, and IGF-I R .
Recombinant Human Decorin
Applications in Research
Recombinant human Decorin is widely used in research to:
- Study ECM assembly and collagen fibrillogenesis: Its ability to modulate collagen fibrillogenesis makes it a valuable tool for understanding ECM dynamics .
- Investigate interactions with growth factors and receptors: Researchers use Decorin to explore its regulatory effects on growth factors and receptor signaling pathways .
- Examine its role in disease models: Decorin’s involvement in various biological processes makes it a subject of interest in studies related to fibrosis, cancer, and other diseases .
Decorin’s multifaceted roles in ECM regulation and its interactions with key biological molecules highlight its importance in both basic and applied research.
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