CYTH3 Human

Cytohesin 3 Human Recombinant
Cat. No.
BT27374
Source
E.coli.
Synonyms
Cytohesin 3, PSCD3, ARNO3, GRP1, PH SEC7 and Coiled-Coil Domain-Containing Protein 3 , Pleckstrin Homology Sec7 and Coiled-Coil Domains 3, General Receptor of Phosphoinositides 1 , ARF Nucleotide-Binding Site Opener 3 , Protein ARNO3 , Cytohesin-3, CYTH3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CYTH3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 422 amino acids (1-399) and having a molecular mass of 48.7 kDa.
CYTH3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cytohesin 3 (CYTH3), a member of the PSCD family, plays a role in protein sorting and membrane trafficking regulation. PSCD family members share a structured organization: an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. CYTH3 is known to regulate Golgi structure and function and may have physiological functions in regulating ADP-ribosylation factor protein 6 (ARF) functions, in addition to its effects on ARF1.
Description
Recombinant human CYTH3, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 422 amino acids (1-399). It has a molecular mass of 48.7 kDa. CYTH3 is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The CYTH3 solution is provided at a concentration of 0.25 mg/ml in a buffer composed of 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 20% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Cytohesin 3, PSCD3, ARNO3, GRP1, PH SEC7 and Coiled-Coil Domain-Containing Protein 3 , Pleckstrin Homology Sec7 and Coiled-Coil Domains 3, General Receptor of Phosphoinositides 1 , ARF Nucleotide-Binding Site Opener 3 , Protein ARNO3 , Cytohesin-3, CYTH3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDEDGGG EGGGVPEDLS LEEREELLDI RRRKKELIDD IERLKYEIAE VMTEIDNLTS VEESKTTQRN KQIAMGRKKF NMDPKKGIQF LIENDLLQSS PEDVAQFLYK GEGLNKTVIG DYLGERDEFN IKVLQAFVEL HEFADLNLVQ ALRQFLWSFR LPGEAQKIDR MMEAFASRYC LCNPGVFQST DTCYVLSFAI IMLNTSLHNH NVRDKPTAER FIAMNRGINE GGDLPEELLR NLYESIKNEP FKIPEDDGND LTHTFFNPDR EGWLLKLGGR VKTWKRRWFI LTDNCLYYFE YTTDKEPRGI IPLENLSIRE VEDPRKPNCF ELYNPSHKGQ VIKACKTEAD GRVVEGNHVV YRISAPSPEE KEEWMKSIKA SISRDPFYDM LATRKRRIAN KK.

Product Science Overview

Introduction

Cytohesin 3, also known as CYTH3, ARNO3, or GRP1, is a protein encoded by the CYTH3 gene in humans . This protein is a member of the cytohesin family, which is characterized by a specific structural organization that includes an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain . These domains are crucial for the protein’s function in cellular processes such as protein sorting and membrane trafficking.

Structural Features
  • N-terminal coiled-coil motif: Involved in homodimerization.
  • Sec7 domain: Contains guanine-nucleotide exchange protein (GEP) activity.
  • PH domain: Interacts with phospholipids and is responsible for the association of CYTHs with membranes .
Function

Cytohesin 3 plays a significant role in the regulation of protein sorting and membrane trafficking. It is involved in the control of Golgi structure and function and may regulate ADP-ribosylation factor protein 6 (ARF6) functions, in addition to acting on ARF1 . The protein promotes guanine-nucleotide exchange on ARF1 and ARF6, facilitating the activation of ARF factors through the replacement of GDP with GTP .

Preparation Methods

Recombinant Human Cytohesin 3 is typically produced in Escherichia coli (E. coli) expression systems. The protein is expressed as a full-length protein, often with a His-tag at the N-terminus to facilitate purification . The purification process involves conventional chromatographic techniques to achieve high purity levels suitable for various applications, including SDS-PAGE .

Applications

Recombinant Cytohesin 3 is used in various research applications, particularly in studies related to cellular signaling, protein-protein interactions, and membrane trafficking. Its ability to promote guanine-nucleotide exchange makes it a valuable tool for investigating ARF-mediated signaling pathways .

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