CYTH2 Human

Cytohesin 2 Human Recombinant
Cat. No.
BT27291
Source
E.coli.
Synonyms
ARF Nucleotide-Binding Site Opener, Pleckstrin Homology Sec7 And Coiled-Coil Domains 2 (Cytohesin-2), PH SEC7 And Coiled-Coil Domain-Containing Protein 2, Cytohesin 2, Protein ARNO, ARF Exchange Factor, Sec7p-Like, PSCD2, PSCD2L, CTS18.1, Sec7p-L, SEC7L.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CYTH2 Human Recombinant produced in E. coli is a single polypeptide chain containing 422 amino acids (1-399) and having a molecular mass of 48.9 kDa. CYTH2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cytohesin 2 (CYTH2) is an ARF-1 guanine nucleotide exchange factor (GEF) that facilitates the exchange of GDP for GTP on ARF proteins. As part of the RAS superfamily, ARF proteins are vital for vesicle budding. CYTH2 specifically activates ARF1, ARF3, and ARF6. Belonging to the PSCD family, CYTH2 plays a role in protein sorting and membrane trafficking regulation. When bound to ARL4 proteins at the cell membrane, it recruits ARF6 to the plasma membrane.
Description
Recombinant human CYTH2, expressed in E. coli, is a single polypeptide chain with a molecular weight of 48.9 kDa. It consists of 422 amino acids, comprising residues 1-399 of the native protein, and includes an N-terminal 23 amino acid His-tag. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
CYTH2 is supplied as a 1 mg/ml solution in 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
ARF Nucleotide-Binding Site Opener, Pleckstrin Homology Sec7 And Coiled-Coil Domains 2 (Cytohesin-2), PH SEC7 And Coiled-Coil Domain-Containing Protein 2, Cytohesin 2, Protein ARNO, ARF Exchange Factor, Sec7p-Like, PSCD2, PSCD2L, CTS18.1, Sec7p-L, SEC7L.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEDGVYE PPDLTPEERM ELENIRRRKQ ELLVEIQRLR EELSEAMSEV EGLEANEGSK TLQRNRKMAM GRKKFNMDPK KGIQFLVENE LLQNTPEEIA RFLYKGEGLN KTAIGDYLGE REELNLAVLH AFVDLHEFTD LNLVQALRQF LWSFRLPGEA QKIDRMMEAF AQRYCLCNPG VFQSTDTCYV LSFAVIMLNT SLHNPNVRDK PGLERFVAMN RGINEGGDLP EELLRNLYDS IRNEPFKIPE DDGNDLTHTF FNPDREGWLL KLGGRVKTWK RRWFILTDNC LYYFEYTTDK EPRGIIPLEN LSIREVDDPR KPNCFELYIP NNKGQLIKAC KTEADGRVVE GNHMVYRISA PTQEEKDEWI KSIQAAVSVD PFYEMLAARK KRISVKKKQE QP.

Product Science Overview

Structure and Function

Cytohesin 2 is a guanine-nucleotide exchange factor (GEF) that promotes the exchange of GDP for GTP on ARF (ADP ribosylation factor) proteins, specifically ARF1, ARF3, and ARF6 . This exchange is crucial for the activation of ARF proteins, which play a central role in vesicle budding and membrane trafficking .

Biological Role

Cytohesin 2 is involved in various cellular processes, including:

  • Protein Sorting and Membrane Trafficking: It mediates the regulation of protein sorting and membrane trafficking .
  • Neurite Growth: It has been implicated in the growth of neurites, which are projections from the cell body of a neuron .
  • Recruitment of ARF6: In association with ARL4 proteins, Cytohesin 2 recruits ARF6 to the plasma membrane .
Recombinant Cytohesin 2

Recombinant Cytohesin 2 is typically produced in Escherichia coli and is available as a full-length protein (1-399 amino acids) with a molecular mass of approximately 48.9 kDa . It is often fused to a His-tag at the N-terminus to facilitate purification . The recombinant protein is used in various applications, including SDS-PAGE and mass spectrometry (MS), and is characterized by a purity greater than 90% .

Applications

Recombinant Cytohesin 2 is used in research to study its role in cellular processes and its interaction with other proteins. It is also utilized in high-throughput screening assays and other biochemical analyses .

Storage and Stability

For optimal stability, recombinant Cytohesin 2 should be stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods. It is recommended to add a carrier protein for long-term storage to prevent multiple freeze-thaw cycles .

Cytohesin 2 plays a vital role in cellular functions and its recombinant form is a valuable tool for scientific research, providing insights into its mechanisms and potential applications in biotechnology and medicine.

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