CST3 Human, Pichia
Pichia pastoris.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 96.0%.
Description
Recombinant Human Cystatin C is having a molecular mass of approximately 13kDa.
Product Specs
Pichia pastoris.
Product Science Overview
Cystatin C is a cysteine proteinase inhibitor that plays a crucial role in regulating proteolytic activity in various physiological processes. It is a small, non-glycosylated protein consisting of 120 amino acids and is produced by all nucleated cells. Cystatin C is considered a valuable biomarker for kidney function and cardiovascular diseases due to its stable production rate and consistent serum levels.
The recombinant production of human Cystatin C in Pichia pastoris involves the use of this yeast as a host organism for protein expressionPichia pastoris is a methylotrophic yeast that is widely used for the production of recombinant proteins due to its ability to perform post-translational modifications, high expression levels, and ease of genetic manipulation .
The gene encoding human Cystatin C is cloned into an expression vector and introduced into Pichia pastoris. The yeast cells are then cultured in a medium containing methanol, which induces the expression of the recombinant protein. The expressed Cystatin C is secreted into the culture medium, from which it can be purified using various chromatographic techniques .
- Medical Diagnostics: Cystatin C is used as a biomarker for assessing kidney function. It is considered more reliable than creatinine-based measurements, especially in detecting early-stage kidney disease.
- Research: Recombinant Cystatin C is used in various research applications to study its role in inhibiting cysteine proteases and its potential therapeutic applications in conditions such as cancer and neurodegenerative diseases .
- Therapeutic Potential: Due to its inhibitory effects on cysteine proteases, Cystatin C has potential therapeutic applications in preventing tissue damage and inflammation in various diseases .
- High Yield: Pichia pastoris can produce high levels of recombinant proteins, making it a cost-effective system for large-scale production.
- Post-Translational Modifications: This yeast can perform post-translational modifications, such as glycosylation, which are essential for the proper function of many proteins.
- Ease of Genetic Manipulation: The genetic tools available for Pichia pastoris allow for easy manipulation and optimization of protein expression .
