Cystatin-C Protein
Escherichia Coli.
Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Cystatin-C Human Recombinant produced in E.Coli is a single, non- glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13.3kDa.
Cystatin-C is purified by proprietary chromatographic techniques.
Product Specs
Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328.
Escherichia Coli.
SSPGKPPRLV GGPMDASVEE EGVRRALDFA VGEYNKASND MYHSRALQVV RARKQIVAGV NYFLDVELGR TTCTKTQPNL DNCPFHDQPH LKRKAFCSFQ IYAVPWQGTM TLSKSTCQDA.
Product Science Overview
Cystatin-C is a low molecular weight protein, approximately 13-14 kDa, and consists of 120 amino acids . It functions primarily as an inhibitor of cysteine proteases, such as cathepsins B, H, K, L, and S, as well as papain . By inhibiting these proteases, Cystatin-C plays a crucial role in regulating proteolytic activity, which is essential for maintaining cellular homeostasis and preventing tissue damage.
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Kidney Function Biomarker: Cystatin-C is widely recognized as a reliable biomarker for assessing kidney function. Unlike creatinine, which can be influenced by muscle mass, age, and gender, Cystatin-C levels are less affected by these factors, making it a more accurate indicator of glomerular filtration rate (GFR) .
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Tumor Suppression: Cystatin-C has been implicated in tumor suppression by inhibiting cathepsin-mediated tumor cell invasion . It also antagonizes TGF-β1 signaling, which is involved in tumor progression .
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Neurological Diseases: Cystatin-C is involved in various neurological conditions. It has been studied for its role in Alzheimer’s disease, where it may inhibit the aggregation of amyloid-beta peptides .
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Inflammation: Cystatin-C is also associated with inflammatory processes. It can modulate antigen presentation by inhibiting cathepsins, which are involved in the processing of antigens .
Recombinant human Cystatin-C is produced using recombinant DNA technology, typically expressed in human cell lines such as HEK293 cells . This recombinant form retains the same biological activity as the native protein and is used in various research and clinical applications.
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Purity and Activity: Recombinant Cystatin-C is highly pure, with a purity level greater than 98% as determined by SDS-PAGE . Its activity is measured by its ability to inhibit papain-mediated cleavage of a fluorogenic substrate .
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Storage and Stability: Recombinant Cystatin-C is stable in its lyophilized form at 4°C for up to one year. Once reconstituted, it should be stored at -20°C to -70°C to maintain its stability and activity .
Recombinant Cystatin-C is used in various research fields, including:
- Nephrology: As a biomarker for kidney function assessment.
- Oncology: To study its role in tumor suppression and cancer progression.
- Neurology: For research on its involvement in neurological diseases.
- Immunology: To understand its role in modulating immune responses and inflammation.
