Cyclophilin B Mouse

Recombinant mouse Cyclophilin-B is typically expressed in Escherichia coli (E. coli) and is often tagged with a His-tag at the N-terminus to facilitate purification. The protein consists of 216 amino acids, with a molecular weight of approximately 22.7 kDa . The amino acid sequence includes a signal sequence that directs the protein to the endoplasmic reticulum (ER), where it is both retained and secreted .

Cyclophilin-B is primarily located within the endoplasmic reticulum and is associated with the secretory pathway. It is known to bind to cells derived from T- and B-lymphocytes and may play a role in regulating cyclosporine A-mediated immunosuppression . The protein’s specific activity is defined as the amount of enzyme that cleaves 1 nmole of suc-AAFP-PNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin .

Recombinant mouse Cyclophilin-B is used in various research applications, including enzyme activity assays and SDS-PAGE. It is also utilized in studies related to protein folding, immunosuppression, and cell signaling .

For short-term storage, Cyclophilin-B can be kept at 4°C. For long-term storage, it is recommended to aliquot and store the protein at -20°C to -80°C to avoid repeated freeze-thaw cycles, which can degrade the protein .