Cyclophilin B Human

Cyclophilin-B is a cyclosporine-binding protein that plays a crucial role in protein folding and trafficking within the ER. It is involved in the secretory pathway and is released in biological fluids . This protein can bind to cells derived from T- and B-lymphocytes and may regulate cyclosporine A-mediated immunosuppression .

Recombinant human Cyclophilin-B is typically expressed in E. coli and purified using conventional chromatography techniques . The recombinant protein corresponds to the amino acids 26-216 of human Cyclophilin-B and is often un-tagged . It has a predicted molecular mass of approximately 21.2 kDa .

Cyclophilin-B has been shown to interact with various cellular components, including the Gag protein, and is involved in p300-mediated degradation of CHOP in tumor cell adaptation to hypoxia . It also possesses proinflammatory properties and plays a principal role in ER redox homeostasis . High levels of Cyclophilin-B are found in inflammatory processes such as sepsis and rheumatoid arthritis .

Cyclophilin-B is identified as a candidate gene for Osteogenesis Imperfecta (OI-IX), an inherited disorder of connective tissue . Mutations in the PPIB gene, which encodes Cyclophilin-B, can lead to defects in collagen production, resulting in brittle bones and other connective tissue abnormalities .

Recombinant Cyclophilin-B is typically stored at -20°C to -80°C under sterile conditions to maintain its stability and biological activity . It is recommended to avoid repeated freeze-thaw cycles to preserve the protein’s integrity .