Cyclophilin B Antibody
Cyclophilin-B, Mouse Anti Human
Cat. No.
BT16118
Source
Synonyms
Peptidylprolyl isomerase B, PPIase, Rotamase, S-cyclophilin, PPIB, cyclophilin-like protein, peptidyl-prolyl cis-trans isomerase B, Cyclophilin B, SCYLP, CYPB, CYP-S1, MGC2224, MGC14109.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
Cyclophilin B, also known as PPIB or peptidylpropyl isomerase B, is a cyclosporine-binding protein primarily found in the endoplasmic reticulum. It plays a role in the secretory pathway and is released into biological fluids. This protein exhibits binding affinity for cells derived from T and B lymphocytes, and may be involved in regulating cyclosporine A-mediated immunosuppression.
Formulation
1 mg/ml in PBS, pH 7.4, with 10% glycerol and 0.02% Sodium Azide.
Storage Procedures
Store at 4°C for up to 1 month. For longer-term storage, keep at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C or 1 month at 4°C.
Applications
This Cyclophilin-B antibody has undergone ELISA and Western blot analysis to confirm its specificity and reactivity. However, optimal working dilutions should be determined experimentally for each application.
Synonyms
Peptidylprolyl isomerase B, PPIase, Rotamase, S-cyclophilin, PPIB, cyclophilin-like protein, peptidyl-prolyl cis-trans isomerase B, Cyclophilin B, SCYLP, CYPB, CYP-S1, MGC2224, MGC14109.
Purification Method
Cyclophilin-B antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
Pk2E2AT.
Immunogen
Anti-human Cyclophilin-B mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Cyclophilin-B amino acids 26-216 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.
Product Science Overview
Biological Function
Cyclophilin B is involved in several cellular processes, including:
- Protein Folding: It assists in the proper folding of newly synthesized proteins within the ER.
- Immune Response: Cyclophilin B is secreted in response to inflammatory stimuli and interacts with CD147, a receptor involved in immune cell activation .
- Calcium Signaling: The CyPB-cyclosporin A complex inhibits calcineurin, a calcium and calmodulin-dependent protein phosphatase, which is crucial for T-cell activation .
Mouse Anti-Human Cyclophilin B Antibody
The Mouse Anti-Human Cyclophilin B Antibody is a monoclonal antibody that specifically targets human Cyclophilin B. This antibody is widely used in various research applications, including:
- Western Blotting (WB): To detect Cyclophilin B in cell lysates .
- Immunohistochemistry (IHC): For staining tissue sections to study the localization of Cyclophilin B .
- Immunocytochemistry (ICC): To visualize Cyclophilin B in cultured cells .
- Flow Cytometry (FC): For analyzing Cyclophilin B expression in different cell populations .
- Enzyme-Linked Immunosorbent Assay (ELISA): To quantify Cyclophilin B levels in various samples .
Applications and Specificity
Research Significance
Cyclophilin B and its antibodies are valuable tools in biomedical research. They help in understanding the role of Cyclophilin B in protein folding, immune response, and calcium signaling. Additionally, studying Cyclophilin B can provide insights into various diseases, including cancer and inflammatory disorders.
For more detailed information, you can refer to the Proteintech and R&D Systems websites.
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