Cyclophilin D Human, His

Cyclophilin-D (Human Recombinant, His Tag) is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain. It consists of 390 amino acids and has a molecular mass of 42.9 kDa . The recombinant protein is fused to a 20 amino acid His Tag at the N-terminus, which facilitates its purification through chromatographic techniques .

Cyclophilin-D possesses PPIase activity and is known to bind to the immunosuppressant cyclosporin-A . This binding is significant because it plays a role in the regulation of mitochondrial permeability transition pore (mPTP), which is crucial for cell death mechanisms such as apoptosis . Overexpression of Cyclophilin-D has been shown to suppress apoptosis in cancer cells through a mitochondrial hexokinase-2 dependent mechanism .

Due to its role in apoptosis and protein folding, Cyclophilin-D is a valuable tool in various research fields, including cancer research and studies on mitochondrial function . The recombinant form, with the His Tag, allows for easy purification and use in laboratory settings .

Cyclophilin-D (Human Recombinant, His Tag) is typically stored at 4°C if it will be used within 2-4 weeks. For longer storage periods, it is recommended to store the protein frozen at -20°C. To ensure stability, it is advisable to add a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) and avoid multiple freeze-thaw cycles .

The recombinant protein is provided as a sterile filtered colorless solution. It is formulated in a solution containing 1x phosphate-buffered saline (PBS) at pH 7.4 and 10% glycerol . The purity of the protein is greater than 95.0% as determined by SDS-PAGE .