Cyclophilin D Human
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The Cyclophilin-D is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Cyclophilin-D is particularly significant due to its involvement in mitochondrial function and apoptosis. It is known to bind to the immunosuppressant cyclosporin-A, which inhibits its activity . Overexpression of Cyclophilin-D has been observed to suppress apoptosis in cancer cells through a mitochondrial hexokinase-2 dependent mechanism . This makes it a potential target for cancer therapy and other medical applications.
Human recombinant Cyclophilin-D is typically produced in Escherichia coli (E. coli). The recombinant protein is a single, non-glycosylated polypeptide chain containing 178 amino acids and has a molecular mass of approximately 18.9 kDa . The purification process involves proprietary chromatographic techniques to ensure high purity and activity .
Cyclophilin-D possesses PPIase activity, which accelerates the folding of proteins and thus their formation . It plays a decisive role in mitochondrial permeability transition, a process crucial for cell survival and apoptosis . The enzyme’s activity is essential for maintaining mitochondrial function and integrity.
Due to its role in protein folding and mitochondrial function, Cyclophilin-D is widely used in laboratory research. It is particularly valuable in studies related to apoptosis, cancer, and mitochondrial diseases . Researchers utilize recombinant Cyclophilin-D to investigate its interactions with other proteins and potential inhibitors, such as cyclosporin-A .
For optimal stability, Cyclophilin-D should be stored at 4°C if used within 2-4 weeks. For longer storage periods, it is recommended to freeze the protein at -20°C and add a carrier protein like 0.1% HSA or BSA to prevent degradation . It is crucial to avoid multiple freeze-thaw cycles to maintain the protein’s activity and integrity .
Cyclophilin-D (Human Recombinant) is a vital tool in biochemical and medical research, offering insights into protein folding, mitochondrial function, and apoptosis. Its production and purification from E. coli ensure a high-quality, active enzyme for various experimental applications.
