Cyclophilin B Human, His
Cyclophilin-B Human Recombinant, His Tag
Cat. No.
BT3035
Source
Escherichia Coli.
Synonyms
Peptidylprolyl isomerase B, PPIase, Rotamase, S-cyclophilin, PPIB, cyclophilin-like protein, peptidyl-prolyl cis-trans isomerase B, Cyclophilin B, SCYLP, CYPB, CYP-S1, MGC2224, MGC14109.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Cyclophilin-B Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Asp34-Glu216) containing 193 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 22kDa.
Product Specs
Introduction
Cyclophilin B, also known as PPIB or peptidylpropyl isomerase B, is a cyclosporine-binding protein found primarily in the endoplasmic reticulum. It plays a role in the secretory pathway and is released into biological fluids. Cyclophilin B can bind to cells from T and B lymphocytes, potentially regulating cyclosporine A-mediated immunosuppression.
Description
Recombinant Human Cyclophilin-B, expressed in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids Asp34 to Glu216 (193 amino acids total) and containing a 10-amino acid His tag at the N-terminus. The calculated molecular mass is 22 kDa.
Physical Appearance
White lyophilized (freeze-dried) powder.
Formulation
Cyclophilin-B was sterile filtered (0.4 µm) and lyophilized in 20 mM Tris buffer with 50 mM NaCl, at pH 7.5.
Solubility
To prepare a working stock solution, add deionized water to the lyophilized pellet to achieve a concentration of approximately 0.5 mg/ml. Allow the pellet to dissolve completely. Note: This product is not sterile. Filter through an appropriate sterile filter before use in cell culture.
Stability
Store lyophilized protein at -20°C. After reconstitution, aliquot the product to avoid repeated freeze-thaw cycles. Reconstituted protein remains stable at 4°C for a limited period (up to two weeks with no observable change).
Purity
Purity is determined to be greater than 95.0% by SDS-PAGE.
Synonyms
Peptidylprolyl isomerase B, PPIase, Rotamase, S-cyclophilin, PPIB, cyclophilin-like protein, peptidyl-prolyl cis-trans isomerase B, Cyclophilin B, SCYLP, CYPB, CYP-S1, MGC2224, MGC14109.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHAS DEKKKGPKVT VKVYFDLRIG DEDVGRVIFG LFGKTVPKTV DNFVALATGE KGFGYKNSKF HRVIKDFMIQ GGDFTRGDGT GGKSIYGERF PDENFKLKHY GPGWVSMANA GKDTNGSQFF ITTVKTAWLD GKHVVFGKVL EGMEVVRKVE STKTDSRDKP LKDVIIADCG KIEVEKPFAI AKE.
Product Science Overview
Structure and Expression
The recombinant human Cyclophilin-B protein with a His tag is typically expressed in HEK293 cells or Escherichia coli. The His tag is a polyhistidine sequence added to the protein to facilitate purification via metal affinity chromatography . The recombinant protein consists of 190 amino acids and has a molecular weight of approximately 22 kDa .
Function and Biological Activity
Cyclophilin-B is both secreted and retained in the endoplasmic reticulum (ER). It plays a crucial role in ER redox homeostasis and has proinflammatory properties. High levels of Cyclophilin-B are found in inflammatory processes such as sepsis and rheumatoid arthritis . Additionally, Cyclophilin-B mediates chemotaxis and T cell adhesion to fibronectin .
Industrial Production and Purification
The production of recombinant Cyclophilin-B involves the following steps:
- Gene Cloning: The DNA sequence encoding the mature form of human PPIB is cloned into an expression vector.
- Expression: The vector is introduced into host cells (HEK293 or Escherichia coli), where the protein is expressed.
- Purification: The His tag allows for purification using nickel affinity chromatography. The protein is then further purified to achieve a purity of over 95% .
Applications
Recombinant Cyclophilin-B is used in various research areas, including:
- Protein Folding Studies: Due to its PPIase activity, it is used to study protein folding mechanisms.
- Inflammation Research: Its role in inflammatory processes makes it a valuable tool for studying diseases like rheumatoid arthritis and sepsis.
- Drug Development: Cyclophilin-B is a target for developing drugs that modulate its activity, potentially leading to new treatments for inflammatory diseases .
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