CYB5A Human, Sf9
Sf9, Baculovirus cells.
Cytochrome b5 isoform 1, CYB5A, CYB5, MCB5, Microsomal cytochrome b5 type A.
Greater than 90.0% as determined by SDS-PAGE.
Description
CYB5A produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 117 amino acids (1-108a.a.) and having a molecular mass of 13.3kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).
CYB5A is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Cytochrome b5 isoform 1, also known as CYB5A, belongs to the cytochrome b5 family and is associated with the cell membrane. This protein plays a crucial role in the activity of stearyl-CoA-desaturase by reducing ferric hemoglobin to ferrous hemoglobin. CYB5A acts as an electron carrier for various oxygenases embedded within the cell membrane.
CYB5A, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 117 amino acids (specifically, amino acids 1 to 108a.a.). Its molecular weight is 13.3kDa. However, on SDS-PAGE analysis, the apparent molecular size ranges from 18kDa to 28kDa. This CYB5A protein is expressed with a 9 amino acid His tag located at the C-terminus. Purification is achieved using proprietary chromatographic methods.
The CYB5A protein solution is provided at a concentration of 1mg/ml. It is prepared in a buffer containing phosphate-buffered saline (pH 7.4) and 10% glycerol.
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage periods, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
The purity of CYB5A is determined to be greater than 90.0% based on SDS-PAGE analysis.
Cytochrome b5 isoform 1, CYB5A, CYB5, MCB5, Microsomal cytochrome b5 type A.
Sf9, Baculovirus cells.
ADPMAEQSDE AVKYYTLEEI QKHNHSKSTW LILHHKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDAREMSK TFIIGELHPDDRPKLNKPPE TLITTIDSSS SHHHHHH.
Product Science Overview
The CYB5A gene is located on chromosome 18 in humans . Cytochrome B5 Type A is predominantly expressed in the liver, where it is involved in the electron transfer to fatty-acid desaturases and cytochrome P450 enzymes . It is also co-expressed in the Leydig cells of the testis and theca cells of the ovary .
Cytochrome B5 Type A mediates several essential biochemical processes :
- NADH-dependent reduction of methemoglobin: This process occurs in erythrocytes.
- Electron transfer: It facilitates electron transfer to fatty-acid desaturases, primarily in the liver.
- Steroid and xenobiotics metabolism: It plays a role in the metabolism of steroids and xenobiotics by cytochrome P450 enzymes.
- Androstenedione synthesis: It is essential for the efficient synthesis of gonadal androgen .
The recombinant form of Cytochrome B5 Type A is often expressed in Sf9 cells (Spodoptera frugiperda) for research purposes . Sf9 cells are a type of insect cell line commonly used in biotechnology for the production of recombinant proteins. The recombinant protein is typically tagged with histidine to facilitate purification and is expressed with high purity (≥90% as determined by SDS-PAGE) .
Recombinant Cytochrome B5 Type A is used in various research applications, including:
- Biochemical studies: To understand its role in electron transfer and interaction with other proteins.
- Drug metabolism research: To study its involvement in the metabolism of pharmaceuticals.
- Steroid biosynthesis: To investigate its role in the synthesis of steroids and other related compounds.
