CYB5A Human
Cytochrome B5 Type A Human Recombinant
Cat. No.
BT26434
Source
Escherichia Coli.
Synonyms
Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CYB5A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids (1-108 a.a) and having a molecular mass of 14.9kDa.
CYB5A is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CYB5A is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Cytochrome b5 isoform 1 (CYB5A) is a member of the cytochrome b5 family. It is a membrane-bound hemoprotein that acts as an electron carrier for several membrane-bound oxygenases. Genetic defects in CYB5A can lead to CYB5A-related methemoglobinemia (METHB-CYB5A). METHB-CYB5A is a type of methemoglobinemia, a blood disorder characterized by an excess of methemoglobin in red blood cells. This excess hinders the blood's oxygen-carrying capacity, resulting in cyanosis and hypoxia.
Description
Recombinant human CYB5A, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 132 amino acids (with amino acids 1 to 108 being the actual CYB5A sequence) and has a molecular weight of 14.9 kDa. A 24-amino acid His-tag is fused to the N-terminus of CYB5A for purification purposes, which is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The CYB5A protein solution is supplied at a concentration of 1 mg/ml. The solution is buffered with 20 mM Tris-HCl at a pH of 8.0 and also contains 20% glycerol, 0.1 M NaCl, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be kept at 4°C. For longer storage, the product should be frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the CYB5A protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAEQSD EAVKYYTLEE IQKHNHSKST WLILHHKVYD LTKFLEEHPG GEEVLREQAG GDATENFEDV GHSTDAREMS KTFIIGELHP DDRPKLNKPP ETLITTIDSS SS.
Product Science Overview
Structure and Isoforms
Cytochrome B5 Type A has two isoforms produced by alternative splicing:
- Isoform 1: This isoform is bound to the cytoplasmic side of the endoplasmic reticulum and contains a C-terminal transmembrane alpha-helix .
- Isoform 2: This isoform is found in the cytoplasm .
The protein’s structure includes a heme-binding domain, which is essential for its electron transfer function. The heme group is coordinated by two histidine residues, providing the fifth and sixth ligands to the iron atom in the heme .
Biological Functions
Cytochrome B5 Type A is involved in several critical biological processes:
- Electron Transport: It acts as an electron donor to various oxygenases, facilitating oxidative reactions .
- Fatty Acid Metabolism: It is essential for the desaturation and elongation of fatty acids, which are vital for maintaining cell membrane integrity and producing signaling molecules .
- Cholesterol Biosynthesis: Cytochrome B5 Type A is involved in the synthesis of cholesterol, a crucial component of cell membranes and precursor for steroid hormones .
- Drug Metabolism: It participates in the metabolism of various drugs and xenobiotics by cytochrome P450 enzymes .
Clinical Significance
Recombinant Production
Human recombinant Cytochrome B5 Type A is typically produced using Escherichia coli expression systems. The recombinant protein is often tagged with histidine to facilitate purification . This recombinant form is used in various research applications to study its structure, function, and interactions with other proteins.
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