PF4 Bovine
CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Platelet Factor-4 (CXCL4) Bovine Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 88 amino acid and having a molecular mass of approximately 9.5kDa.
PF4 is purified by proprietary chromatographic techniques.
Product Specs
Platelet factor-4 (PF4) is a protein found in the granules of platelets. When platelets are activated, they release PF4. This protein plays a role in blood clotting by binding to a molecule called heparin. PF4 is also involved in inflammation and wound healing. Oncostatin-A, another protein, is similar in structure to PF4. This protein has been investigated for its potential to inhibit the formation of new blood vessels, a process that is important in tumor growth.
This product is a recombinant version of bovine Platelet Factor-4 (CXCL4) produced in E. coli bacteria. It is a single, non-glycosylated polypeptide chain composed of 88 amino acids with a molecular weight of approximately 9.5 kDa. The protein has been purified using proprietary chromatographic techniques.
The protein was lyophilized from a concentrated solution (0.2 μm filtered) in 20 mM phosphate buffer (PB) and 500 mM sodium chloride (NaCl) at pH 7.0.
To reconstitute the lyophilized Platelet Factor-4, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 μg/ml. The reconstituted solution can be further diluted in other aqueous solutions.
Lyophilized CXCL4 can be stored at room temperature for up to 3 weeks, but for long-term storage, it is recommended to store it in a desiccated state below -18°C. Once reconstituted, Platelet Factor-4 should be stored at 4°C for a maximum of 2-7 days. For long-term storage, it can be stored below -18°C. Avoid repeated freeze-thaw cycles to maintain protein stability.
The purity of the protein is greater than 95.0% as determined by:
(a) High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
The biological activity of the protein was determined using a chemotaxis assay with human neutrophils. The effective concentration range for inducing chemotaxis in this assay is between 10-100 ng/ml.
CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.
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Product Science Overview
Platelet Factor-4 (PF4), also known as CXCL4, is a small cytokine belonging to the CXC chemokine family. It is released from the alpha granules of activated platelets during the process of platelet aggregation. PF4 plays a crucial role in various physiological and pathological processes, including coagulation, inflammation, and wound healing .
PF4 is a non-glycosylated polypeptide chain containing 88 amino acids and has a molecular mass of approximately 9.5 kDa . It forms a homotetramer with a high affinity for heparin, which allows it to neutralize heparin-like molecules on the endothelial surface of blood vessels, thereby promoting blood coagulation . Additionally, PF4 is chemotactic for neutrophils and monocytes and functions as an inhibitor of hematopoiesis, angiogenesis, and T-cell function .
Recombinant PF4 (rPF4) is produced using various expression systems, with Escherichia coli (E. coli) being one of the most commonly used hosts. The production process involves the insertion of the PF4 gene into a plasmid vector, which is then introduced into E. coli cells. The recombinant protein is expressed and subsequently purified using chromatographic techniques .
Recombinant PF4 has several applications in research and clinical settings. It is used in the diagnosis of heparin-induced thrombocytopenia (HIT), a condition where antibodies form against PF4-heparin complexes, leading to a decrease in platelet count . Additionally, rPF4 is utilized in studies investigating its role in various diseases, including liver fibrosis and cancer .
