CXCL17 Rat

VCC1 is a secreted molecule with a size and predicted 3-dimensional folding pattern similar to that of chemokines CXCL8/IL8 and CXCL14/BRAK . It is constitutively expressed in the lung and can also be detected in the trachea, stomach, and skeletal muscle . The mature Rat CXCL17 shares 82% and 71% amino acid sequence identity with mouse and human CXCL17, respectively .

VCC1 plays a significant role in the chemotaxis of quiescent peripheral blood monocytes and dendritic cells, but not LPS-activated ones . It specifically binds to these cells and induces their migration. The expression of VCC1 is increased in endothelial cells when they are induced to form tubes in vitro . This chemokine is also involved in angiogenesis, showing significantly correlated expression with VEGF in primary lung, breast, and esophageal tumors . Therefore, VCC1 is suggested to have a role in tumor angiogenesis.

Recombinant VCC1 is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain containing 98 amino acids and having a molecular mass of 11.5 kDa . The recombinant protein is purified using proprietary chromatographic techniques and is available as a sterile filtered white lyophilized (freeze-dried) powder .

Lyophilized VCC1 is stable at room temperature for three weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, it should be stored at 4°C for 2-7 days and for future use below -18°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) to prevent freeze-thaw cycles .

Recombinant VCC1 is used for research purposes, including as a positive control, immunogen, and in assays such as SDS-PAGE and Western Blot (WB) . It is important to note that this material is offered for research use only and is not intended for clinical diagnostic procedures .