CXCL17 Human

VEGF Co-regulated Chemokine 1 Human Recombinant
Cat. No.
BT10362
Source
Escherichia Coli.
Synonyms
VEGF coregulated chemokine 1, C-X-C motif chemokine 17, Dendritic cell and monocyte chemokine-like protein, DMC, CXCL17, VCC1, Dcip1, VCC-1, UNQ473.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CXCL17 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 98 amino acids and having a molecular mass of 11.5kDa.
The CXCL17 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Dendritic cell and monocyte chemokine-like protein (DMC/CXCL17/VEGF-correlated chemokine 1/VCC1) is a secreted molecule structurally similar to chemokines CXCL8/IL8 and CXCL14/BRAK. Primarily produced by airway and intestinal epithelium, CXCL17 selectively attracts inactive peripheral blood monocytes and dendritic cells. This chemokine also demonstrates increased expression in endothelial cells during tube formation. Notably, CXCL17, alongside angiogenesis-related chemokines CXCL1/GRO and CXCL8/IL8, exhibits correlated expression with VEGF in lung, breast, and esophageal tumors, suggesting a potential role in tumor angiogenesis. Sequence analysis reveals a high degree of conservation across species, with the mature Rat CXCL17 sharing 82% and 71% amino acid similarity with mouse and human CXCL17, respectively.
Description
Recombinant human CXCL17, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 98 amino acids. This protein, with a molecular weight of 11.5 kDa, is purified using advanced chromatographic techniques.
Physical Appearance
White, lyophilized powder, sterile-filtered.
Formulation
The lyophilized CXCL17 protein was prepared in a 0.2µm filtered solution of phosphate-buffered saline (PBS) at pH 7.4, supplemented with 3% trehalose.
Solubility
To reconstitute, dissolve the lyophilized CXCL17 in sterile 18 MΩ·cm water to a concentration of at least 100 µg/ml. Further dilutions can be prepared in other aqueous solutions.
Stability
While the lyophilized CXCL17 remains stable at room temperature for up to 3 weeks, storage in desiccated conditions below -18°C is recommended. After reconstitution, the protein is stable at 4°C for 2-7 days. For prolonged storage, freeze at -18°C, ideally with a carrier protein like 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0% as determined by: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis. (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The half-maximal effective concentration (ED₅₀), determined by the chemokine's ability to induce VEGF expression in murine endothelial cells, is less than 5.0 µg/ml. This corresponds to a specific activity greater than 200 IU/mg.
Synonyms
VEGF coregulated chemokine 1, C-X-C motif chemokine 17, Dendritic cell and monocyte chemokine-like protein, DMC, CXCL17, VCC1, Dcip1, VCC-1, UNQ473.
Source
Escherichia Coli.
Amino Acid Sequence
SSLNPGVARG HRDRGQASRR WLQEGGQECE CKDWFLRAPR RKFMTVSGLP KKQCPCDHFK GNVKKTRHQR HHRKPNKHSR ACQQFLKQCQ LRSFALPL.

Product Science Overview

Introduction

CXCL17 is strategically expressed in mucosal tissues and plays a significant role in the immune response. It is involved in the recruitment of immune cells, such as monocytes and dendritic cells, to sites of inflammation . This chemokine is also known for its broad antimicrobial activity, which includes disrupting bacterial membranes .

Preparation Methods

Recombinant human CXCL17 is typically produced using Escherichia coli (E. coli) expression systems . The protein is expressed as a fusion protein and then purified through various chromatographic techniques to achieve high purity levels. The recombinant protein is often lyophilized and can be reconstituted in sterile phosphate-buffered saline (PBS) for use in various applications .

Chemical Reactions Analysis

CXCL17 has been shown to induce the expression of vascular endothelial growth factor (VEGF) in endothelial cells, which is crucial for angiogenesis . This chemokine also upregulates the RNA levels of genes involved in angiogenesis, including VEGF-A and basic fibroblast growth factor (FGF) . Additionally, CXCL17 can induce the migration of CD14+ monocytes and CD11c+ immature dendritic cells from human peripheral blood .

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