CTSS Mouse
Description
CTSS is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
EQLQRDP TLDYHWDLWK KTHEKEYKDK NEEEVRRLIW EKNLKFIMIH NLEYSMGMHT YQVGMNDMGD MTNEEISCRM GALRISRQSP KTVTFRSYSN RTLPDTVDWR EKGCVTEVKY QGSCGACWAF SAVGALEGQL KLKTGKLISL SAQNLVDCSN EEKYGNKGCG GGYMTEAFQY IIDNGGIEAD ASYPYKAMDE KCHYNSKNRA ATCSRYIQLP FGDEDALKEA VATKGPVSVG IDASHSSFFF YKSGVYDDPS CTGNVNHGVL VVGYGTLDGK DYWLVKNSWG LNFGDQGYIR MARNNKNHCG IASYCSYPEI LEHHHHHHDY WLVKNSWGLN FGDQGYIRMA RNNKNHCGIA
SYCSYPEILE HHHHHH.
Product Science Overview
Cathepsin-S (CTSS) is a lysosomal cysteine protease belonging to the papain family. It plays a crucial role in various physiological and pathological processes, including immune responses, tumor metastasis, and neurodegenerative diseases. Recombinant mouse Cathepsin-S is a valuable tool for studying these processes in a controlled laboratory setting.
Cathepsin-S is synthesized as an inactive precursor consisting of 331 amino acids, including a 15-amino acid signal peptide, a 99-amino acid propeptide, and a 217-amino acid mature polypeptide . The enzyme is activated in the lysosomes through proteolytic cleavage of the propeptide, resulting in the mature, active form of the enzyme .
Cathepsin-S has several important physiological functions:
- Antigen Presentation: It is critical for the proteolytic digestion of the invariant chain chaperone molecules, which control antigen presentation to CD4+ T-cells by major histocompatibility complex (MHC) class II molecules . It also participates in the direct processing of exogenous antigens for presentation by MHC class II to CD4+ T-cells or in cross-presentation by MHC class I molecules to CD8+ T-cells .
- Immune Response: Cathepsin-S is involved in the secretion of pro-inflammatory cytokines and the regulation of Ca²⁺ flux homeostasis . Suppression of Cathepsin-S leads to elevated levels of anti-inflammatory cytokines and changes in Ca²⁺ influx .
- Neurogenesis and Cognitive Function: Recent studies have shown that targeting Cathepsin-S can enhance cognitive function by modulating the Ca²⁺ influx and activating the brain-derived neurotrophic factor (BDNF)/TrkB axis .
Recombinant mouse Cathepsin-S is produced using DNA sequences encoding the full length of mouse CTSS (AAB94925.1) and expressed in HEK293 cells . The recombinant protein consists of 334 amino acids and has a predicted molecular mass of 37.6 kDa . It is supplied in a sterile buffer and is stable for up to twelve months when stored at -20°C to -80°C .
Recombinant mouse Cathepsin-S is used in various research applications, including:
- Studying Antigen Presentation: It helps in understanding the mechanisms of antigen processing and presentation by MHC molecules.
- Investigating Immune Responses: It is used to study the role of Cathepsin-S in immune responses and inflammation.
- Exploring Neurogenesis: It aids in research on cognitive function and neurogenesis, particularly in the context of aging and neurodegenerative diseases.
