CTSD Human
HEK293 Cells
Description
CTSD produced in HEK293 cells is a single, glycosylated polypeptide chain containing 398 amino acids (21-412 a.a.) and having a molecular mass of 43.4kDa. CTSD is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
HEK293 Cells
LVRIPLHKFT SIRRTMSEVG GSVEDLIAKG PVSKYSQAVP AVTEGPIPEV LKNYMDAQYY GEIGIGTPPQ CFTVVFDTGS SNLWVPSIHC KLLDIACWIH HKYNSDKSST YVKNGTSFDI HYGSGSLSGY LSQDTVSVPC QSASSASALG GVKVERQVFG EATKQPGITF IAAKFDGILG MAYPRISVNN VLPVFDNLMQ QKLVDQNIFS FYLSRDPDAQ PGGELMLGGT DSKYYKGSLS YLNVTRKAYW QVHLDQVEVA SGLTLCKEGC EAIVDTGTSL MVGPVDEVRE LQKAIGAVPL IQGEYMIPCE KVSTLPAITL KLGGKGYKLS PEDYTLKVSQ AGKTLCLSGF MGMDIPPPSG PLWILGDVFI GRYYTVFDRD NNRVGFAEAA RL-HHHHHH
> 20 pmol/min/ug, defined as the amount of enzyme which cleaves 1pmol of Mca-PLGLDpa-AR-NH2/min at pH-3.5 at 25C.
Product Science Overview
Cathepsin-D is composed of a protein dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor . The enzyme is ubiquitously distributed in lysosomes and is involved in the breakdown of various proteins, including amyloid-β protein (Aβ) and the microtubule-associated protein tau . These proteins are significant in the context of neurodegenerative diseases such as Alzheimer’s disease.
Cathepsin-D has been implicated in the pathogenesis of several diseases, including breast cancer and Alzheimer’s disease . In Alzheimer’s disease, Cathepsin-D degrades both Aβ and tau, which accumulate pathognomonically in the disease . Genetic deletion of Cathepsin-D in mice has been shown to trigger large increases in cerebral Aβ and tau, leading to prominent tauopathy . This suggests that Cathepsin-D plays a major role in the proteostasis of these proteins in vivo.
Recombinant human Cathepsin-D is produced using various expression systems, such as HEK 293 cells . This recombinant form is used in research to study the enzyme’s function and its role in disease. The recombinant protein retains the same structure and function as the native enzyme, making it a valuable tool for scientific studies.
