CTGF Human (183-255)
HEK293 cells.
CCN2, NOV2, HCS24, IGFBP8, MGC102839, CTGF, Connective Tissue Growth Factor.
Greater than 95.0% as determined by SDS-PAGE.
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Description
CTGF Human Recombinant is a single, glycosylated polypeptide chain containing 80 amino acids (183-255a.a) and having a molecular mass of 9.1kDa (calculated). CTGF is fused to a 7 a.a His tag at N-terminal.
Product Specs
Connective Tissue Growth Factor (CTGF), a member of the CCN protein family, plays a crucial role in regulating various cellular functions like proliferation, differentiation, and survival. This family, comprising six members in humans, is involved in the development of multiple tissues and organs, including the kidney, nervous system, and bone. CTGF's involvement in wound healing, bone repair, and disease pathologies such as tumor development and fibrosis highlights its significance. While the full-length protein may exhibit antiproliferative effects, truncated isoforms potentially act as oncogenes, promoting proliferation. The protein's structure consists of four modules, each with specific functions. Notably, Module III contributes to cell adhesion, while Module IV appears crucial for several biological activities. Overexpression of CTGF has been linked to various cancers, including invasive mammary ductal carcinomas and glioblastoma. Elevated CTGF levels correlate with higher malignancy grades in chondrosarcomas. In astrocytomas, CTGF influences cell proliferation, with its downregulation leading to growth arrest. Moreover, increased CTGF presence in acute lymphoblastic leukemia patients emphasizes its potential role in disease progression.
Recombinant Human CTGF is a single, glycosylated polypeptide chain consisting of 80 amino acids (183-255a.a). With a molecular weight of 9.1kDa (calculated), it includes a 7 a.a His tag at the N-terminal.
The CTGF protein undergoes filtration (0.4 μm) and lyophilization from a solution of 0.5mg/ml in 20 mM Tris buffer and 50 mM NaCl, at a pH of 7.5.
To create a working stock solution, add deionized water to the lyophilized pellet, aiming for a concentration of approximately 0.5mg/ml. Allow the pellet to dissolve completely.
For long-term storage, keep the lyophilized protein at -20°C. After reconstituting the protein, aliquot it into smaller portions to minimize freeze-thaw cycles, which can degrade the protein. Reconstituted protein remains stable at 4°C for a limited period; no significant changes are observed for up to two weeks at this temperature.
The purity of the protein is greater than 95.0%, as assessed by SDS-PAGE analysis.
CCN2, NOV2, HCS24, IGFBP8, MGC102839, CTGF, Connective Tissue Growth Factor.
HEK293 cells.
MHHHHHHRLE DTFGPDPTMI RANCLVQTTE WSACSKTCGM GISTRVTNDN ASCRLEKQSR LCMVRPCEAD LEENIKKGKK.
Product Science Overview
CTGF is structurally characterized by having four conserved, cysteine-rich domains :
- Insulin-like Growth Factor Binding Protein (IGFBP) Domain: This domain is involved in binding insulin-like growth factors.
- Von Willebrand Type C Repeats (vWC) Domain: This domain is important for protein-protein interactions.
- Thrombospondin Type 1 Repeat (TSR) Domain: This domain interacts with vascular endothelial growth factor (VEGF).
- C-terminal Domain (CT): This domain contains a cysteine knot motif and interacts with members of the TGF-β superfamily, fibronectin, perlecan, fibulin-1, slit, and mucins.
CTGF exerts its functions by binding to various cell surface receptors in a context-dependent manner, including integrin receptors, cell surface heparan sulfate proteoglycans (HSPGs), LRPs, and TrkA . Additionally, CTGF binds to growth factors and extracellular matrix proteins. For example:
- The N-terminal half of CTGF interacts with aggrecan.
- The TSR domain interacts with VEGF.
- The CT domain interacts with members of the TGF-β superfamily and other extracellular matrix proteins.
CTGF is critically involved in fibrotic diseases and several forms of cancer . Knockout mice with the Ctgf gene disrupted die at birth due to respiratory stress caused by severe chondrodysplasia . These mice also show defects in angiogenesis, with impaired interaction between endothelial cells and pericytes and collagen IV deficiency in the endothelial basement membrane . CTGF is also important for pancreatic beta cell development and normal ovarian follicle development and ovulation .
CTGF is associated with wound healing and virtually all fibrotic pathology . It is thought that CTGF can cooperate with TGF-β to induce sustained fibrosis and exacerbate extracellular matrix production in association with other fibrosis-inducing conditions . Overexpression of CTGF in fibroblasts promotes fibrosis in the dermis, kidney, and lung .
The human recombinant CTGF (183-255 a.a.) is a specific fragment of the full-length CTGF protein. This fragment retains some of the functional domains and is used in various research and therapeutic applications . It is produced using recombinant DNA technology, which involves inserting the gene encoding the CTGF fragment into a host organism, such as bacteria or yeast, to produce the protein in large quantities .
