CTGF Human, His

Connective Tissue Growth Factor (CTGF) is part of the CCN protein family, which includes six members in humans: Cyr61 (Cystein rich 61), CTGF, Nov (Nephroblastoma Overexpressed gene), and WISP-1, 2, and 3 (Wnt-1 Induced Secreted Proteins). These secreted proteins interact with the Extracellular Matrix (ECM) and cell membranes, playing a crucial role in regulating cell functions like growth, specialization, survival, adhesion, and movement. Found in tissues derived from all three embryonic layers, CCN proteins are essential for the development of various organs, including the kidneys, nervous system, muscles, bone marrow, cartilage, and bones. In adults, they contribute to wound healing, bone fracture repair, and are implicated in conditions like fibrosis, vascular diseases, and tumor development. Full-length CCN proteins can inhibit cell growth, while shorter versions may promote it and act as oncogenes. Structurally, these proteins consist of four modules. Module I shares similarities with Insulin-like Growth Factor Binding Proteins (IGFBPs). Module II contains a region resembling the Von Willebrand Factor Type C repeat (VWC). Module III includes a Thrombospondin type 1 repeat (TSP1)-like sequence, potentially involved in cell adhesion. Module IV, or CT, is crucial for various CCN protein functions and shares similarities with domains in proteins like Von Willebrand factor and mucins. Breakdown of full-length CCN proteins, observed in CCN2 and CCN3, might produce peptides with a strong affinity for ligands that full-length CCN proteins bind weakly. While shortened CCN2 versions show biological activity, this hasn't been observed in truncated CCN3. Increased CCN2 expression is linked to various cancers, including invasive mammary ductal carcinomas. Studies show a correlation between CCN2 expression and the aggressiveness of chondrosarcomas. In brain tumors, CCN2 is found in both tumor cells and blood vessel-forming cells, suggesting a role in tumor angiogenesis. In astrocytomas, high-grade tumors exhibit elevated CCN2 levels, impacting cell proliferation. Reducing CCN2 expression in these cells leads to growth arrest, while overexpressing it boosts cell numbers in the G1 phase. Research suggests that around 50 genes might be involved in CCN2's proliferative effects. Additionally, CCN2 is found in higher amounts in the immune cells of patients with acute lymphoblastic leukemia.

Recombinant Human CTGF, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 344 amino acids (27-349) with a molecular weight of 37.7kDa. This protein includes a 21 amino acid His-Tag at the N-terminus and is purified using specialized chromatographic techniques.

A clear solution that has been sterilized by filtration.

CTGF protein is supplied at a concentration of 1mg/ml in a solution containing 20mM Tris-HCl (pH 8) and 10% Glycerol.

For short-term storage (up to 4 weeks), the entire vial can be stored at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing.

The purity is determined to be greater than 85% based on SDS-PAGE analysis.

CCN2, NOV2, HCS24, IGFBP8, MGC102839, CTGF, Connective Tissue Growth Factor.

Escherichia Coli.

MGSSHHHHHH SSGLVPRGSH MQNCSGPCRC PDEPAPRCPA GVSLVLDGCG CCRVCAKQLG ELCTERDPCD PHKGLFCDFG SPANRKIGVC TAKDGAPCIF GGTVYRSGES FQSSCKYQCT CLDGAVGCMP LCSMDVRLPS PDCPFPRRVK LPGKCCEEWV CDEPKDQTVV GPALAAYRLE DTFGPDPTMI RANCLVQTTE WSACSKTCGM GISTRVTNDN ASCRLEKQSR LCMVRPCEAD LEENIKKGKK CIRTPKISKP IKFELSGCTS MKTYRAKFCG VCTDGRCCTP HRTTTLPVEF KCPDGEVMKK NMMFIKTCAC HYNCPGDNDI FESLYYRKMY GDMA.