CRP Canine
HEK293 cells.
C-reactive protein, CRP, PTX1, MGC88244, MGC149895.
Greater than 95.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
CRP Canine Recombinant is a single, glycosylated polypeptide chain containing 204 amino acids and having a molecular mass of 23.2kDa (calculated).
Product Specs
C-reactive protein (CRP) is an acute-phase protein produced by the liver. Its levels rise significantly in response to inflammation caused by various factors like bacterial infections, rheumatoid arthritis, viral infections, and other inflammatory conditions. CRP serves as a valuable marker for monitoring inflammation. Notably, CRP levels show a strong correlation with Serum Amyloid A levels.
Recombinant Canine CRP is a single polypeptide chain that contains 204 amino acids. It has a molecular weight of 23.2 kDa. This protein is glycosylated, meaning it has sugar molecules attached to it.
CRP is supplied as a lyophilized powder, having been filtered through a 0.4 µm filter. The protein was initially in a solution of 20mM Tris buffer, 50mM NaCl, and 1mM CaCl2 at a pH of 7.5, with a concentration of 0.5mg/ml, before being lyophilized.
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To prepare a working stock solution, add deionized water to the lyophilized pellet to achieve a concentration of approximately 0.5mg/ml. Allow the pellet to fully dissolve.
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Store the lyophilized protein at -20°C. After reconstituting the protein, divide it into smaller portions (aliquots) to prevent repeated freezing and thawing cycles. The reconstituted protein remains stable at 4°C for a limited period; no significant changes are observed after two weeks at this temperature.
The purity of the CRP protein is greater than 95%, as determined by SDS-PAGE analysis.
C-reactive protein, CRP, PTX1, MGC88244, MGC149895.
HEK293 cells.
QIDLDEKAFV FPRESENSYV ILFPQLQKPM KAFTVCLQVY TDLTRPHSLF SYATKSQSNE ILLFKERPGL FSVSVGGSDA FINFPQKFYA PQHFCVTWES VTGLTELWVD GKPMVRASLR RGYTVGSGAS IVLGQEQDSF GGGFDKNQSL VGDIEDVNMW DFVLSPSQIL TLYTTRALSP NVLNWRNLRY ETRGEVFLKK ELWS
Product Science Overview
C-Reactive Protein (CRP) is a major acute-phase reactant synthesized primarily in the liver hepatocytes. It is a pentraxin (cyclic pentameric protein) composed of five identical nonglycosylated subunits of 206 amino acids each, which are bound noncovalently to form the physiologic CRP molecule . CRP mediates activities associated with preimmune nonspecific host resistance, acting as an opsonin, an initiator of the classical complement cascade, and an activator of monocytes/macrophages .
In dogs, CRP is produced by the liver in response to inflammation and can be reliably measured in canine serum . Circulating concentrations of CRP increase within 24 hours of tissue injury in dogs and may be used as both a prognostic indicator and a management tool for various inflammatory diseases . CRP levels typically begin to decline 18 to 24 hours after the initiation of appropriate treatment or mitigation of the inciting cause .
Recombinant Canine CRP is produced using a eukaryotic expression system and consists of 204 amino acids . This recombinant protein is used in various research and diagnostic applications to study inflammation and immune responses in dogs. The recombinant form allows for consistent and controlled studies, providing valuable insights into the role of CRP in canine health.
CRP plays a crucial role in the innate immune response by binding to several nuclear components, including chromatin, histones, and small nuclear ribonucleoproteins (snRNPs), suggesting that it may act as a scavenger during cell necrosis . It is also involved in the opsonization of pathogens, facilitating their recognition and clearance by phagocytic cells .
CRP is a sensitive marker of inflammation in dogs and is used in veterinary practice to diagnose and monitor various conditions, including infections, immune-mediated diseases, neoplasia, pneumonia, discospondylitis, and pancreatitis . The measurement of CRP levels can aid in the initial assessment of patients and in determining the response to therapy .
