CRISP3 Human
Cysteine-Rich Secretory Protein 3 Human Recombinant
Cat. No.
BT26288
Source
Escherichia Coli.
Synonyms
Cysteine-Rich Secretory Protein 3, Specific Granule Protein of 28 KDa, CRISP-3, SGP28, Cysteine-Rich Secretory Protein-3, Specific Granule Protein (28 KDa), DJ442L6.3, CRS3, Aeg2, CRISP3.
Appearance
Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CRISP3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (21-245) containing 235 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 26.74kDa.
Product Specs
Introduction
Cysteine-rich secretory protein 3 (CRISP3) belongs to the CRISP protein family. It comprises a pathogenesis related protein 1 (PR1) domain at its N-terminal, an ion channel regulatory (ICR) domain at its C-terminal, and a connecting hinge region. CRISP3 transcripts are primarily found in the prostate. While CRISP3 expression remains low in benign prostatic epithelium, it is significantly upregulated in most high-grade prostatic intraepithelial neoplasia lesions, primary prostate tumors, and metastases. Notably, CRISP3 is frequently upregulated in prostate cancer. Human Prostate Secretory Protein (PSP94) exhibits a high affinity for human CRISP3.
Description
Recombinant human CRISP3, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 235 amino acids (21-245). It includes a 10 amino acid His tag located at the N-terminus. The calculated molecular mass of this protein is 26.74 kDa.
Physical Appearance
A clear, colorless solution after filtration.
Formulation
CRISP3 solution, filtered through a 0.4 µm membrane, at a concentration of 0.3 mg/ml in a buffer composed of 50 mM acetate (pH 4), 5 mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity determined by SDS-PAGE is greater than 85.0%.
Synonyms
Cysteine-Rich Secretory Protein 3, Specific Granule Protein of 28 KDa, CRISP-3, SGP28, Cysteine-Rich Secretory Protein-3, Specific Granule Protein (28 KDa), DJ442L6.3, CRS3, Aeg2, CRISP3.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHASNEDKDPAFTA LLTTQTQVQR EIVNKHNELR RAVSPPARNM LKMEWNKEAA ANAQKWANQC NYRHSNPKDR MTSLKCGENL YMSSASSSWS QAIQSWFDEY NDFDFGVGPK TPNAVVGHYT QVVWYSSYLV GCGNAYCPNQ KVLKYYYVCQ YCPAGNWANR LYVPYEQGAP CASCPDNCDD GLCTNGCKYE DLYSNCKSLK LTLTCKHQLV RDSCKASCNC SNSIY.
Product Science Overview
Structure and Domains
Expression and Function
CRISP-3 is expressed in various tissues, including the male and female reproductive tracts, where it plays roles in sperm function, fertilization, and endometrial receptivity for embryo implantation . It is also found in mammalian exocrine secretions and granulocytes, suggesting a role in innate immunity .
Clinical Significance
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